13TSB_ACT: Evaluation of MALDI-TOF-MS to differentiate sub species of the pathogen S uberis to provide an improved approach to mastitis control

13TSB_ACT：评估 MALDI-TOF-MS 区分乳房链球菌病原体亚种，以提供改进的乳腺炎控制方法

• 批准号: BB/M005119/1
• 负责人: Martin Green
• 金额: $ 15.36万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM005119%2F1
• 关键词: 13TSB_ACT; Evaluation; MALDI; TOF; MS

Bovine mastitis is an inflammatory disease of the udder and is a major barrier to the sustainability of dairy farmingworldwide. The health, welfare, economic and environmental impacts of bovine mastitis are severe. It is the most commoncause of on-farm death and premature culling. With an incidence rate of clinical mastitis of 60/100 cows/year more thanone million cows are affected per annum in the UK alone. Mastitis treatment and control accounts for over 30% of allantibiotic use in dairy cattle. Annual losses exceed £170M in the UK; 38% of the total direct costs of disease. Thereforemastitis substantially reduces the efficiency of dairy farming and an increased rate of culling of mastitic animals adverselyaffects the environment and the drive towards national and international food security, while antibiotic use in cattle mayalso spread resistance to bacteria that impact on human health.Mastitis is caused by a bacterial infection; the bacteria enter the mammary gland via the teat canal. The central premise ofmastitis control is to minimise the risk of infection by reducing transmission of bacterial pathogens into the udder. In orderto control mastitis, it is necessary to understand how and when bacterial infections occur and how the pathogens cause aninfection. Although only a few species of bacteria commonly cause bovine mastitis, it is now recognised that sub species(strains) of these bacteria behave in different ways in terms of disease transmission and outcome. The aim of this researchis to develop a method to differentiate strains of the most common mastitis pathogen, S uberis, according to their diseasetransmission characteristics. To do this, a modern, high throughput method of mass spectrometry (matrix-assisted laserdesorption ionization/time-of-flight mass spectrometry - MALDI-TOF-MS) will be used to differentiate strains of S uberis.These strains will be grouped according to how they cause mastitis and the outcome of disease. This information will thenbe used to inform mastitis control programmes which will include reduced use of antibiotics on farm.If successful, his research will pride a new and improved approach to mastitis control in dairy cows. This would be to thebenefit of dairy farmers (reduced disease costs), veterinary surgeons and advisors (improved ability to advise on mastitiscontrol), the dairy industry (improved sustainability, welfare and image by reducing mastitis) and consumers (improved cowwelfare and food security).

牛乳腺炎是一种乳房炎症性疾病，是全球奶牛养殖可持续发展的主要障碍。牛乳腺炎对健康、福利、经济和环境的影响是严重的。这是农场死亡和过早扑杀的最常见原因。临床乳腺炎的发病率为 60/100 头奶牛/年，仅在英国每年就有超过 100 万头奶牛受到影响。乳腺炎的治疗和控制占奶牛所有抗生素使用量的 30% 以上。英国每年损失超过 1.7 亿英镑；疾病直接成本总额的 38%。因此，乳腺炎大大降低了奶牛养殖的效率，增加了乳腺动物的扑杀率，对环境以及国家和国际粮食安全的推动产生了不利影响，而牛身上使用抗生素也可能传播对影响人类健康的细菌的耐药性。乳腺炎是由细菌感染引起的；乳腺炎是由细菌感染引起的。细菌通过乳头管进入乳腺。控制乳腺炎的核心前提是通过减少细菌病原体传播到乳房来最大程度地降低感染风险。为了控制乳腺炎，有必要了解细菌感染如何以及何时发生以及病原体如何引起感染。虽然只有少数几种细菌通常会引起牛乳腺炎，但现在人们认识到这些细菌的亚种（菌株）在疾病传播和结果方面表现不同。本研究的目的是开发一种方法，根据最常见的乳腺炎病原体乳房链球菌的疾病传播特征来区分它们的菌株。为此，将使用现代高通量质谱方法（基质辅助激光解吸电离/飞行时间质谱 - MALDI-TOF-MS）来区分乳房链球菌菌株。这些菌株将根据其引起乳腺炎的方式和疾病结果进行分组。然后，这些信息将用于为乳腺炎控制计划提供信息，其中包括减少农场抗生素的使用。如果成功，他的研究将为奶牛乳腺炎控制提供一种新的、改进的方法。这将有利于奶农（降低疾病成本）、兽医和顾问（提高乳腺炎控制建议的能力）、乳制品行业（通过减少乳腺炎提高可持续性、福利和形象）和消费者（改善奶牛福利和粮食安全）。

项目成果

Molecular Epidemiology of Streptococcus uberis Clinical Mastitis in Dairy Herds: Strain Heterogeneity and Transmission.

奶牛群乳房链球菌临床乳腺炎的分子流行病学：菌株异质性和传播。

• DOI: 10.1128/jcm.01583-15
• 发表时间: 2016-01
• 期刊: Journal of clinical microbiology
• 影响因子: 9.4
• 作者: Davies PL;Leigh JA;Bradley AJ;Archer SC;Emes RD;Green MJ
• 通讯作者: Green MJ

Prediction of Streptococcus uberis clinical mastitis risk using Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) in dairy herds.

使用基质辅助激光解吸时间在乳制品牛群中的飞行质谱（MALDI-TOF MS）的链球菌临床乳腺炎预测。

• DOI: 10.1016/j.prevetmed.2017.05.015
• 发表时间: 2017-09-01
• 期刊: Preventive veterinary medicine
• 影响因子: 2.6
• 作者: Archer SC;Bradley AJ;Cooper S;Davies PL;Green MJ
• 通讯作者: Green MJ

Discrimination of contagious and environmental strains of Streptococcus uberis in dairy herds by means of mass spectrometry and machine-learning.

通过质谱和机器学习的乳制群中，乳球链球菌的传染性和环境菌株的歧视。

• DOI: 10.1038/s41598-018-35867-6
• 发表时间: 2018-11-30
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Esener N;Green MJ;Emes RD;Jowett B;Davies PL;Bradley AJ;Dottorini T
• 通讯作者: Dottorini T