14TSB_SynBio: BaseHunter: a software tool for synthetic biology procurement

蛋白质通过四种化学碱基（A、T、C和G）编码成DNA。由三个碱基组成的一串叫做密码子。由于dna有四个碱基，因此最多可以形成64个（4x4x4）唯一密码子。在64个可能的密码子中，61个用于编码氨基酸。只有20种不同的氨基酸用于构建蛋白质。生命不是使用未使用的密码子，而是使用平均三个不同的密码子编码相同氨基酸的系统。因此，相同的蛋白质序列可以被许多不同的DNA序列编码。每个DNA序列在翻译效率、转录效率、蛋白质折叠和易合成性等参数方面会有所不同。代码优化是一种算法过程，旨在为给定目的选择最佳可能的变体。该项目的研究部分旨在开发一种方法来衡量不同密码子优化算法的性能。这将通过获得不同的密码子优化基因并测量它们在生命系统中的行为来实现。

Proteins are encoded into DNA using four chemical bases (A, T, C and G). A string of three bases is called a codon. SinceDNA has four bases, a maximum of 64 (4x4x4) unique codons can be formed. Of the 64 possible codons, 61 are used toencode amino acids. There only 20 different amino acids used to construct proteins. Rather than having unused codons,life uses a system in which an average of three different codons encode the same amino acid. Consequently, the sameprotein sequence can be encoded with many different DNA sequences. Each DNA sequence will vary with respect toparameters such as translational efficiency, transcriptional efficiency, protein folding and ease of synthesis. Codonoptimisation is an algorithmic process that seeks to pick the best possible variant for a given purpose. The research part ofthis project seeks to develop a way of measuring how well different codon optimisation algorithms perform. This will bedone by obtaining different codon optimised genes and measuring how they behave in living systems.