MULTIPLE DRUG RESISTANCE IN HUMAN SOLID TUMORS

多药耐药（Multiple Drug Resistance，MDR）是肿瘤治疗失败的主要原因 化疗早期对小鼠细胞系的研究已经确定了某些 生物化学机制（例如快速药物外排）导致MDR。 细胞标记物如细胞表面糖蛋白通常与 关于MDR我们对实体瘤MDR的了解有限， 研究人类实体肿瘤的耐药性很重要，因为 不，MDR导致治疗失败。 在本研究中，我们计划研究人实体瘤细胞的MDR 分离自患者的腹水和胸膜液以及异种移植物 早先建立的。我们将使用我们的激光激发方法来排序， 根据蒽环类药物的荧光含量来研究肿瘤细胞。 将在暴露或不暴露的情况下研究药物转运特征 药物外排阻断剂。生物化学机制负责MDR以及 将研究众所周知的MDR标志物。 希望拟议的研究将提供有关机制的见解， 参与人实体瘤的MDR。

Multiple Drug Resistance (MDR) is a major reason for failure of cancer chemotherapy. Earlier studies on murine cell lines have identified certain biochemical mechanisms (e.g. rapid drug efflux) responsible for MDR. Cellular markers such as cell surface glycoproteins are often associated with MDR. Our knowledge about MDR in solid tumors is limited and it is important to study drug resistance in human soild tumors, as more often than not, MDR results in treatment failure. In the present study, we propose to study MDR in human solid tumor cells isolated from ascites and pleural fluid of patients and from xenografts earlier established. We will use our laser excitation method to sort and study tumor cells on the basis of their anthracycline fluorescence content. Drug transport characteristics will be studied with or without exposure to drug efflux blockers. Biochemical mechanisms responsible for MDR as well as well-known markers of MDR will be studied. It is hoped that the studies proposed will provide insights into mechanisms involved in MDR of human solid tumors.