14 TSB-ACT-1R Biotechnology for Anti-Weeds (BAW)

14 TSB-ACT-1R 抗杂草生物技术 (BAW)

• 批准号: BB/M012867/1
• 负责人: Viacheslav Tverezovskiy
• 金额: $ 61.04万
• 依托单位: Bangor University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM012867%2F1
• 关键词: 14; TSB; ACT; 1R; Biotechnology

The project aim is to establish an economic technology to support a UK-based supply chain for the production of the novel herbicide Sorgoleone. There is currently no green "nature identical" herbicide on the market, and the major classes of commercially available products are now in the mature phase of their life-cycle. Sorgoleone, a powerful allelopathic chemical, is currently produced by plants in minute concentration. This project seeks to develop a commerically viable biotech synthesis route using a plant based starting material (available in thousands of tonnes). The project will develop a UK-based supply chain aimed at producing and selling the chemical. The lead organisation, Hockley International, an UK based SME in the agrochemical market has developed, through a TSB funded KTP, a methodology for the synthesis of the saturated component of sorgoleone using a widely available natural resorcinolic lipid. Hockley has now a patented approach to selectively extract the resorcinolic precursor of the target anti-weed molecule from cashew nut shell liquid (a non-valorised sub-product of the cashew kernel production available in 100,000 tons). Hockley's current chemical process is a five step route involving an oxidation with chromium. This funding will allow investigation into the possible reduction of the five steps to a two or three-step route via a cytochrome P450 enzymatic oxidation and enzymatic methylation. Field testing of the nature-identical synthetic molecules will also be undertaken to estimate its efficacy and applications rates.

该项目的目的是建立一种经济的技术，以支持英国生产新型除草剂Sorgoleone的供应链。目前市场上还没有绿色“天然相同”的除草剂，并且主要类别的市售产品现在处于其生命周期的成熟阶段。Sorgoleone是一种强大的化感作用化学物质，目前由植物以微小浓度产生。该项目旨在开发一种生物技术合成路线，使用基于植物的起始材料（可获得数千吨）。该项目将开发一个以英国为基地的供应链，旨在生产和销售该化学品。牵头组织Hockley International是一家位于英国的农业化学品市场中小企业，通过TSB资助的KTP开发了一种使用广泛可用的天然间苯二酚脂质合成山梨醇酮饱和组分的方法。Hockley现在拥有一种专利方法，可以从腰果壳液（腰果仁生产的一种非增值子产品，产量为100，000吨）中选择性地提取目标抗杂草分子的间苯二酚前体。霍克利目前的化学工艺是一个五步路线，涉及铬的氧化。这笔资金将允许研究通过细胞色素P450酶促氧化和酶促甲基化将五个步骤减少到两个或三个步骤的可能性。还将对天然相同的合成分子进行实地测试，以估计其功效和应用率。

项目成果

Estimating the success of enzyme bioprospecting through metagenomics: current status and future trends.

• DOI: 10.1111/1751-7915.12309
• 发表时间: 2016-01
• 期刊: Microbial biotechnology
• 影响因子: 5.7
• 作者: Ferrer M;Martínez-Martínez M;Bargiela R;Streit WR;Golyshina OV;Golyshin PN
• 通讯作者: Golyshin PN