13TSB_N4L2: CELLDEX - Development of a low calorie bulk sugar replacer

在TSB CELLDEX项目中，RINH （U. Aberdeen）的微生物学小组将执行工作包3的关键部分（WP3.1-“体外人类结肠细菌纤维素糊化性评估”）。他们将使用体外方法评估人体肠道微生物对纤维素糊精的可发酵性，并预测纤维素糊精对微生物群落组成和代谢输出的可能影响。纤维素糊精将在厌氧条件下与混合的人类粪便微生物群一起培养，使用批培养和ph控制的连续花发酵模型。分子方法将用于监测微生物群落组成的变化。发酵的主要产物，短链脂肪酸和气体，将通过气相色谱法监测，纤维素糊精的利用将通过分析总糖来监测。我们实验室从人类结肠中分离出的一系列优势多糖利用厌氧菌以纤维素糊精作为唯一能量来源生长的能力也将在这里进行评估。结合分子群落分析结果，这将提供可能对纤维素糊精利用负责的物种的信息。这项工作将先于诺丁汉大学计划进行的人类饮食干预研究。

The Microbiology Group at RINH (U. Aberdeen) will perform a key element (WP3.1- "Assessment of cellodextrinfermentability by human colonic bacteria in vitro") of Work package 3 within the TSB project CELLDEX. They will use invitro approaches to assess the fermentability of cellodextrins by human intestinal microorganisms and to predict the likelyimpact of cellodextrins upon microbial community composition and metabolic outputs. Cellodextrins will be incubated withmixed human faecal microbiota under anaerobic conditions using batch cultures and pH-controlled continuous flowfermentor models. Molecular approaches will be used to monitor changes in microbial community composition. The majorproducts of fermentation, short chain fatty acids and gases, will be monitored by gas chromatography and cellodextrinutilization will be monitored by analysis of total sugars. The ability of a range of dominant polysaccharide-utilizinganaerobes that have been isolated in our laboratory from from the human colon to grow with cellodextrins as a sole energysource will also be assessed here. Together with the results from molecular community analysis, this should provideinformation on species likely to be responsible for cellodextrin utilization. This work will precede and inform the plannedhuman dietary intervention studies to be performed by University of Nottingham.