13TSB_N4L2: CELLDEX - Development of a low calorie bulk sugar replacer

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The Microbiology Group at RINH (U. Aberdeen) will perform a key element (WP3.1- "Assessment of cellodextrinfermentability by human colonic bacteria in vitro") of Work package 3 within the TSB project CELLDEX. They will use invitro approaches to assess the fermentability of cellodextrins by human intestinal microorganisms and to predict the likelyimpact of cellodextrins upon microbial community composition and metabolic outputs. Cellodextrins will be incubated withmixed human faecal microbiota under anaerobic conditions using batch cultures and pH-controlled continuous flowfermentor models. Molecular approaches will be used to monitor changes in microbial community composition. The majorproducts of fermentation, short chain fatty acids and gases, will be monitored by gas chromatography and cellodextrinutilization will be monitored by analysis of total sugars. The ability of a range of dominant polysaccharide-utilizinganaerobes that have been isolated in our laboratory from from the human colon to grow with cellodextrins as a sole energysource will also be assessed here. Together with the results from molecular community analysis, this should provideinformation on species likely to be responsible for cellodextrin utilization. This work will precede and inform the plannedhuman dietary intervention studies to be performed by University of Nottingham.