13TSB_N4L2: CELLDEX - Development of a low calorie bulk sugar replacer

13TSB_N4L2：CELLDEX - 开发低热量散装糖替代品

• 批准号: BB/M013766/1
• 负责人: Harry Flint
• 金额: $ 6.26万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM013766%2F1
• 关键词: 13TSB_N4L2; CELLDEX; Development; low; calorie

The Microbiology Group at RINH (U. Aberdeen) will perform a key element (WP3.1- "Assessment of cellodextrinfermentability by human colonic bacteria in vitro") of Work package 3 within the TSB project CELLDEX. They will use invitro approaches to assess the fermentability of cellodextrins by human intestinal microorganisms and to predict the likelyimpact of cellodextrins upon microbial community composition and metabolic outputs. Cellodextrins will be incubated withmixed human faecal microbiota under anaerobic conditions using batch cultures and pH-controlled continuous flowfermentor models. Molecular approaches will be used to monitor changes in microbial community composition. The majorproducts of fermentation, short chain fatty acids and gases, will be monitored by gas chromatography and cellodextrinutilization will be monitored by analysis of total sugars. The ability of a range of dominant polysaccharide-utilizinganaerobes that have been isolated in our laboratory from from the human colon to grow with cellodextrins as a sole energysource will also be assessed here. Together with the results from molecular community analysis, this should provideinformation on species likely to be responsible for cellodextrin utilization. This work will precede and inform the plannedhuman dietary intervention studies to be performed by University of Nottingham.

Rinh(U.Aberdeen)的微生物学小组将在TSB项目Celldex中执行工作包3的一个关键要素(WP3.1--“体外人类结肠细菌纤维糊精可转移性的评估”)。他们将使用体外方法来评估人体肠道微生物对纤维糊精的发酵性，并预测纤维糊精对微生物群落组成和代谢产出的可能影响。纤维糊精将与混合的人类粪便微生物群在厌氧条件下使用分批培养和pH控制的连续流动发酵模型进行培养。将使用分子方法来监测微生物群落组成的变化。发酵的主要产物，短链脂肪酸和气体，将通过气相色谱进行监测，纤维糊精的利用将通过总糖分析进行监测。我们实验室从人的结肠中分离出的一系列主要利用多糖的厌氧菌以纤维糊精为唯一能源的能力也将在这里进行评估。结合分子群落分析的结果，这应该提供有关可能负责纤维糊精利用的物种的信息。这项工作将在诺丁汉大学计划进行的人类饮食干预研究之前进行，并为其提供信息。