The use of protein surfactants as formulation ingredients

使用蛋白质表面活性剂作为配方成分

• 批准号: BB/M013774/1
• 负责人: Cait MacPhee
• 金额: $ 23.85万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM013774%2F1
• 关键词: use; protein; surfactants; formulation; ingredients

Bacteria are single celled organisms that live in social communities called biofilms. Over the millennia bacteria have evolved sophisticated strategies to introduce three-dimensionality to the biofilm. The architecturally complex structure is generated biologically using a combination of proteins and large sugar molecules that function in the environment outside the cell. One protein that is needed for biofilm formation by a bacterium called Bacillus subtilis is BslA. This protein can self-assemble into an elastic film and forms a hydrophobic "raincoat" over the cells in the biofilm protecting them from external insults. In this application we will exploit nature by using the inherent self-assembly and hydrophobic properties of BslA within a wide range of applications of high industrial significance.

细菌是单细胞生物，生活在称为生物膜的社会社区中。数千年来，细菌已经进化出复杂的策略，将三维引入生物膜。这种结构复杂的结构是由蛋白质和在细胞外环境中发挥作用的大糖分子组合在生物学上产生的。一种被称为枯草芽孢杆菌的细菌形成生物膜所需的蛋白质是BslA。这种蛋白质可以自组装成弹性膜，并在生物膜中的细胞上形成疏水性“雨衣”，保护它们免受外部侵害。在本申请中，我们将利用自然界中的固有自组装和疏水性能的BslA在广泛的应用范围内的高工业意义。

项目成果

Formation of functional, non-amyloidogenic fibres by recombinant Bacillus subtilis TasA.

• DOI: 10.1111/mmi.13985
• 发表时间: 2018-12
• 期刊: Molecular microbiology
• 影响因子: 3.6
• 作者: Erskine E;Morris RJ;Schor M;Earl C;Gillespie RMC;Bromley KM;Sukhodub T;Clark L;Fyfe PK;Serpell LC;Stanley-Wall NR;MacPhee CE
• 通讯作者: MacPhee CE

BslA-stabilized emulsion droplets with designed microstructure.

• DOI: 10.1098/rsfs.2016.0124
• 发表时间: 2017-08-06
• 期刊: Interface focus
• 影响因子: 4.4
• 作者: Bromley KM;MacPhee CE
• 通讯作者: MacPhee CE

Connecting the dots between bacterial biofilms and ice cream.

连接细菌生物膜和冰淇淋之间的点。

• DOI: 10.1088/1478-3975/12/6/063001
• 发表时间: 2015
• 期刊: Physical biology
• 影响因子: 2
• 作者: Stanley-Wall NR