BONE MARROW TRANSPLANTATION FOR HIV PATIENTS WITH CANCER

该项目要求支持临床前和临床研究 评估骨髓移植(BMT)的作用和 抗病毒化疗在L感染者治疗中的应用 患有恶性血液病。提出了三个具体目标。 在目标1中，我们计划确定是否随后进行强化治疗 通过骨髓移植可以消除L感染艾滋病毒的细胞和如果 3‘-叠氮-3’-脱氧胸腺嘧啶核苷(AZT)可预防猪瘟病毒感染 从异基因骨髓中获取的移植供体细胞。在……里面 目的2，我们计划确定哪些淋巴造血细胞 HIV-L阳性患者携带病毒情况判断 干细胞中存在病毒，以及细胞的比例是多少？ L感染艾滋病不同阶段患者的临床研究 疾病。在目标3中，我们计划调查多能性 不感染艾滋病毒的淋巴造血干细胞--L可以用EX产生 活体骨髓操作和“净化”技术。如果这个 AIM是成功的，这将导致考虑自体骨髓移植 作为对艾滋病病毒感染者L的治疗。

This project requests support for preclinical and clinical studies evaluating the role of bone marrow transplantation (BMT) and antiviral chemotherapy in the treatment of HIV-l positive patients with hematologic malignancy. Three specific aims are addressed. In Aim 1, we plan to determine whether intensive therapy followed by BMT can eliminate the reservoir of HIV-l infected cells and if 3'-azido-3'deoxythymidine (AZT) can prevent infection of transplanted donor cells obtained from allogeneic bone marrow. In Aim 2, we plan to determine which lympho-hematopoietic cells in HIV-l positive patients carry the virus to determine whether the virus is present in stem cells and what fraction of cells are infected with HIV-l in patients at different stages of their disease. In Aim 3, we plan to investigate whether pluripotent lympho-hematopoietic stems free of HIV-l can be generated with ex vivo bone marrow manipulation and "purging" techniques. If this aim is successful it will lead to consideration of autologous BMT as therapy for patients with HIV-l infection.