ULTRASTRUCTURAL HISTOPATHOLOGY OF HUMAN DENTAL ENAMEL

这项研究目前关注的是利用关于 人牙釉质发育过程中的超微结构形态和性质 前几年的这个项目，以获得对珐琅的了解 畸形、釉质晶体溶解和釉质修复。我们的长期合作 假设釉质晶体的成核，生长，取向， 和排列(成棱镜)依赖于 结晶到有机基质和成釉细胞。我们的目标是澄清 这些釉质组织的超微结构排列和排列紊乱 发育和成熟的正常和病理状态的成分 珐琅。TEM结果将与其他观测结果直接相关 和技术[例如临床、接触式显微放射照相(CMR)、光 显微镜(LM)和扫描电子显微镜(SEM)]。我们的特定 目的是(A)利用发育中的大鼠磨牙诱导的成釉细胞下囊肿 作为检查超微结构牙釉质组织病理学的有用模型，以及(B) 确定牙釉质修复的最佳治疗方法 牙釉质组织的超微结构 脱矿/再矿化。 大鼠磨牙-氟化钠发育中的成釉细胞下囊肿--钠盐研究 氟化物诱发牙囊肿和牙釉质缺陷将延期至 回收过程，评估表面，确定密度和晶体大小 分泌期和成熟期。二膦酸盐--重点研究 分泌和成熟过程中的细胞效应与直接晶体效应 各阶段。氧气水平-新生大鼠将处于低氧环境中 测定单独和联合作用对大鼠牙釉质发育的影响 含NaF和二磷酸盐。 成熟的人牙釉质-脱矿，再矿化，有缺陷： 二膦酸盐.表面效应.使用2二膦酸盐的实验 (EHDP，Cl2MDP)在成熟的釉质表面上，酸蚀前后。 扫描电子显微镜的结果将补充CMS和透射电子显微镜。 脱矿/再矿化/龋齿--牛的初步实验 牙釉质将被重新定向到对成熟的人牙釉质进行整体治疗。 变化将通过CMR和TEM进行评估，并与不稳定和 正常的牙釉质。釉质簇状物-持续酸(TCA)和EDTA(pH 7.2) 将对实验进行修改，以确定剩余部分的分布 水晶。组织病理学.含牙釉质的乳牙和恒牙 缺陷将被检查，并与对照组和大鼠磨牙进行比较 实验。

The research is currently concerned with utilizing the information on the ultrastructural morphology and properties of tooth enamel developed in previous years of this project to gain an understanding of enamel malformation, enamel crystal dissolution, and enamel repair. Our long-term hypothesis is that the enamel crystal's nucleation, growth, orientation, and arrangement (into prisms) is dependent on a unique relationship of the crystal to the organic matrix and the ameloblast. It is our aim to clarify the ultrastructural arrangement and disarrangement of these enamel tissue components in normal and pathological states in developing and mature enamel. TEM results will be directly correlated with other observations and techniques [e.g. clinical, contact microradiography (CMR), light microscopy (LM), and scanning electron microscopy (SEM)]. Our specific aims are (a) using the subameloblastic cyst induced in developing rat molar as a useful model to examine ultrastructural enamel histopathology, and (b) determining optimal treatments for enamel repair by clarifying the ultrastructure of enamel tissue especially during demineralization/remineralization. Subameloblastic Cysts in Developing Rat Molar - NaF - studies using sodium fluoride to induce cysts and defective enamel will be extended to follow recovery process, evaluate surface, determine density and crystal size at secretory and maturation stages. Diphosphonates - studies focused on cellular versus direct crystal effects during secretory and maturation stages. O2 levels - newborn rats will be in a reduced oxygen atmosphere to determine effects of rat enamel development both alone and in combination with NaF and diphosphonates. Mature Human Enamel - Demineralized, Remineralized, Defective: Diphosphonates - Surface Effects - experiments using 2 diphosphonates (EHDP, Cl2MDP) on mature enamel surfaces, before and after acid etching. SEM results will be supplemented with CMS and TEM. Demineralization/Remineralization/Caries - initial experiments with bovine enamel will be redirected to en bloc treatment of mature human enamel. Changes will be evaluated by CMR and TEM and compared with precarious and normal enamel. Enamel Tufts - ongoing acid (TCA) and EDTA (pH 7.2) experiments will be modified to determine distribution of remaining crystals. Histopathology - deciduous teeth and permanent teeth with enamel defects will be examined and compared to controls and to rat molar experiments.