MECHANISMS OF SUGAR TRANSPORT IN STREPTOCOCCUS MUTANS

变形链球菌的糖转运机制

• 批准号: 3219675
• 负责人: GARY R JACOBSON
• 金额: $ 9.31万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-06-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219675
• 关键词: Streptococcus mutans; carbohydrate metabolism; carbohydrate transport; dental caries; enzyme structure; glucose; microorganism culture; microorganism metabolism; oral bacteria; phosphorylation; phosphotransferases; scintillation counter; sucrose

The sucrose phosphotransferase system (PTS) is one means by which the oral pathogen Streptococcus mutans takes up this disaccharide from the environment. Because sucrose metabolism has been linked to the cariogenicity of S. mutans, our laboratory has been studying the biochemistry of the PTS in this organism. Our long-range goal is to understand the mechanism and regulation of the sucrose PTS in S. mutans to be able to identify important factors influencing the activity of this transport system in vivo. Our work also may lead to the identification of specific inhibitors of sucrose uptake and growth of S. mutans. In continuing this project, we aim to: 1) purify Enzyme I and the sucrose-specific enzyme III of the S. mutans PTS to apparent homogeneity and investigates their properties; 2) purify the sucrose-specific Enzyme II from S. mutans membranes; 3) further investigate sucrose analogs as inhibitors of the sucrose PTS and any other sucrose transport systems of S. mutans; 4) determine the physiological significance of ATP-dependent phosphyorylations of S. mutans proteins; and 5) continue 31p-NMR studies as a complementary approach toward understanding sugar transport systems and their regulation in S. mutans. A combination of well-accepted biochemical and biophysical approaches will be used to accomplish these aims.

蔗糖磷酸转移酶系统（PTS）是口服给药的一种手段， 病原体变形链球菌从 环境 因为蔗糖代谢与 S.我们的实验室一直在研究 这种生物体内PTS的生物化学。 我们的长远目标是 了解S.变异到 能够确定影响这一活动的重要因素 体内运输系统。 我们的工作也可能导致识别 蔗糖吸收和生长的特异性抑制剂。变异体 在 继续这个项目，我们的目标是：1）纯化酶I和 蔗糖特异性酶III。表观同质性变异PTS 并研究了它们的性质; 2）纯化蔗糖特异性酶II 来自酿脓链球菌3）进一步研究蔗糖类似物作为 蔗糖PTS和S. 4）确定ATP依赖性的生理意义 S.变形蛋白;和5）继续31 p-NMR研究， 理解糖运输系统的补充方法， 它们在S.变异人 结合了公认的生化 生物物理学方法将用于实现这些目标。