MECHANISMS OF SUGAR TRANSPORT IN STREPTOCOCCUS MUTANS

蔗糖磷酸转移酶系统(PTS)是口服 病原体变形链球菌从细菌中吸收这种双糖 环境。因为蔗糖新陈代谢与 对于变形链球菌的致龋性，我们实验室一直在研究 这种生物体中的PTS的生物化学。我们的长期目标是 了解变形链球菌蔗糖PTS的作用机制及调控 能够确定影响这一活动的重要因素 体内的运输系统。我们的工作也可能导致对 变形链球菌蔗糖摄取和生长的特异性抑制物。在……里面 继续这个项目，我们的目标是：1)纯化酶I和 变形链球菌PTS蔗糖特异酶III的表观均一性 2)蔗糖专一性酶II的纯化 从变形链球菌的膜中提取；3)进一步研究蔗糖类似物 蔗糖PTS和任何其他蔗糖运输系统的抑制剂。 4)确定ATP依赖的生理意义 变形链球菌蛋白的磷酸化；以及5)继续31P-核磁共振研究，如 了解糖运输系统和糖运输系统的补充方法 他们对变形链球菌的规定。一种广为接受的生物化学组合 生物物理方法将被用来实现这些目标。

The sucrose phosphotransferase system (PTS) is one means by which the oral pathogen Streptococcus mutans takes up this disaccharide from the environment. Because sucrose metabolism has been linked to the cariogenicity of S. mutans, our laboratory has been studying the biochemistry of the PTS in this organism. Our long-range goal is to understand the mechanism and regulation of the sucrose PTS in S. mutans to be able to identify important factors influencing the activity of this transport system in vivo. Our work also may lead to the identification of specific inhibitors of sucrose uptake and growth of S. mutans. In continuing this project, we aim to: 1) purify Enzyme I and the sucrose-specific enzyme III of the S. mutans PTS to apparent homogeneity and investigates their properties; 2) purify the sucrose-specific Enzyme II from S. mutans membranes; 3) further investigate sucrose analogs as inhibitors of the sucrose PTS and any other sucrose transport systems of S. mutans; 4) determine the physiological significance of ATP-dependent phosphyorylations of S. mutans proteins; and 5) continue 31p-NMR studies as a complementary approach toward understanding sugar transport systems and their regulation in S. mutans. A combination of well-accepted biochemical and biophysical approaches will be used to accomplish these aims.