H-2-LINKED CONTROL OF STEROID SUSCEPTIBILITIES

H-2 连锁的类固醇敏感性控制

• 批准号: 3221129
• 负责人: DAVID L GASSER
• 金额: $ 10.7万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3221129
• 关键词: animal population genetics; biological polymorphism; cleft palate; cortisone; genetic disorder; genetic mapping; genetic recombination; genetic strain; histocompatibility gene; immunosuppression; linkage mapping; lymphopenia; major histocompatibility complex; sex hormones; steroid hormone

This project is designed to yield new information about an H-2-linked gene which affects the susceptibility of mice to cortisone-induced cleft palate. We propose to map this gene more precisely by testing a number of congenic strains of mice which have H-2 chromosomes that are recombinants between high and low susceptibility haplotypes. Preliminary evidence suggests that the gene maps between H-2K and the T locus, so experiments have been designed to analyze this genetic region closely. DNA polymorphisms that have been mapped to the region between H-2K and T will be studied in congenic lines that are informative with respect to cleft palate susceptibility. There is also evidence that an H-2-linked gene affects the susceptibility of fetal mice to the masculinizing and feminizing effects of sex steroids. We propose to map this gene more precisely, and to determine whether it differs from the gene which controls cleft palate susceptibility. Finally, we propose to determine whether the susceptibility of mice to sex steroid - induced lymphocyte depletion is affected by H-2-linked genes.

该项目旨在产生关于H-2连锁基因的新信息 这会影响小鼠对可的松诱导的裂缝的敏感性 上颚 我们建议通过测试一些 具有重组H-2染色体的同类小鼠品系 高和低易感性单倍型之间的差异。 初步证据 表明该基因定位在H-2K和T位点之间，因此实验 被设计来仔细分析这个基因区域。 DNA 已经定位到H-2K和T之间区域的多态性将 在关于裂的信息的同类系中进行研究 腭敏感性 也有证据表明，H-2连锁基因影响易感性 性类固醇的雄性化和雌性化效应。 我们建议更精确地绘制这个基因，并确定它是否 与控制腭裂易感性的基因不同。 最后， 我们建议确定小鼠对性类固醇的敏感性- 诱导的淋巴细胞耗竭受H-2连锁基因的影响。