MEASUREMENTS OF ENAMEL PERMEABILITY AS RELATED TO CARIES

一种灵敏的测量口腔内脱矿化和再矿化的方法 牙釉质将被用来测量牙齿的脱矿潜力 食物。该方法涉及使用牛牙釉质块在 假体的构造允许正常的食物消耗。这些区块是 覆盖在人类口腔内的一层致龋性生物体。 有机体暴露在可发酵环境中所产生的酸 碳水化合物导致牙釉质脱矿，很容易被检测到是一种 牙釉质对碘的渗透性增加。液体和液体的测试 固体食物在2小时内就能吃完。唾液中的糖分浓度会 在测试期间确定，并与 脱矿分数。测试将包括生的和糊化的 在实验室准备的淀粉和实验饼干。白糖 和饼干的淀粉含量将被测定，部分 含有标准量碳水化合物的饼干将在 一个测试。曲奇采用瘦肉配方，并用淀粉代替 面粉中的面筋含量、糖粉比和面筋含量。 蛋白质、脂肪和灰分将逐步改变。最终的食谱 将对配方进行测试，目的是确定每个配方的效果 饼干成分对唾液清净率和釉质的影响 去矿物质。不同增稠剂在食品中的应用效果 工业上生产的脱盐蔗糖也将被 调查过了。在其他实验中，碳水化合物的消耗量将是 其次是进食水果和蔬菜或用 用于研究缓解脱矿效果的水 和加速口腔清除。

A sensitive method for measuring intraoral de- and remineralization of tooth enamel will be employed to measure the demineralization potential of food. The method involves the use of blocks of bovine enamel carried on a prosthesis constructed to permit normal food consumption. The blocks are covered by a layer of cariogenic organisms indogenous to the human mouth. The acid generated by the organisms upon exposure to fermentable carbohydrates causes demineralization of the enamel, readily detected as an increase in permeability of the enamel to iodide. Tests of liquid and solid food are completed within 2h. Salivary concentrations of sugar will be determined during the period of the test and correlated with the demineralization score. The tests will include raw and gelatinized starches and experimental cookies prepared in the laboratory. The sugar and starch content of the cookies will be determined, and portions of the cookie containing a standard amount of carbohydrate will be consumed during a test. Employing a lean formula for the cookie, and substituting starch and gluten for wheat flour, the ratio of sugar to starch and the content of protein, fat and ash will be changed step-wise. Eventually cookbook formulae will be tested, with the purpose of determining the effect of each cookie ingredient on the rate of salivary clearance and enamel demineralization. The effect of various thickening agents used in the food industry on the demineralization produced by sucrose will also be investigated. In additional experiments carbohydrate consumption will be followed by consumption of fruits and vegetables or rinsing the mouth with water for the purpose of studying alleviating effects on demineralization and acceleration of oral clearance.