GLYCOCONJUGATES IN OLFACTORY CELL-CELL INTERACTIONS

嗅觉细胞间相互作用中的糖缀合物

• 批准号: 3217714
• 负责人: GERALD A SCHWARTING
• 金额: $ 20.27万
• 依托单位: EUNICE KENNEDY SHRIVER CTR MTL RETARDATN
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3217714
• 关键词: affinity chromatography; axon; carbohydrate structure; cell cell interaction; developmental neurobiology; electron microscopy; enzyme activity; enzyme linked immunosorbent assay; gas chromatography mass spectrometry; gene expression; glycolipids; glycoproteins; glycosyltransferase; hybridomas; immunocytochemistry; laboratory mouse; laboratory rat; lectin; light microscopy; monoclonal antibody; nervous system regeneration; neuronal guidance; neurons; olfactions; olfactory lobe; respiratory epithelium; sensory receptors; sialyltransferases; surface antigens; synaptogenesis

The objectives of this proposal are to understand the role of a specific group of cell surface glycolipids and glycoproteins in cell-cell interactions in olfactory receptor cells. It is designed to test the hypothesis that cell surface carbohydrate antigens interact with ligands on adjacent cell surfaces or on extracellular structures resulting in functionally relevant cellular interactions in the developing, mature and regenerating olfactory system. Three glycoconjugates were recently described which are specifically expressed on subsets of olfactory sensory neurons and their axons. Because of their unique distribution, all three glycoconjugates are candidate cell-cell interaction molecules which may participate in the establishment and maintenance of a functional olfactory system. Two of these molecules in particular may participate in mechanisms which guide olfactory axons to their targets in the olfactory bulb. A third glycoconjugate is expressed on olfactory neurons during an intermediate stage of their maturation and is likely to be relevant for studies of new olfactory connections in adult animals. In the proposed studies, the carbohydrate structures will be chemically characterized using chromatographic and mass spectrometric techniques. Carbohydrate binding proteins which are believed to participate in these cellular interaction will also be identified and chemically characterized. The detailed localization of these glycoconjugates and binding proteins will be analyzed during embryonic and postnatal development using immunocytochemical and electron microscopic techniques. In addition regeneration studies will be conducted to analyze the expression of the glycoconjugates during maturation of olfactory neurons. Glycosyltransferase studies will also be performed in order to understand the regional and age-related differences in expression of maturation-dependent glycoconjugates. This proposal represents a multifaceted approach, combining chemical, cellular, and anatomical analyses to define the role of a group of unique molecules to the olfactory system. These studies will provide insight into the molecular and cellular basis of olfactory structure and function and may illuminate mechanisms of pathogenesis in degenerative sensory disorders.

本提案的目标是了解一个特定的角色