DEVELOPMENT OF EFFERENT NERVE FIBERS IN THE COCHLEA

获得沟通技能的基础是 听力正常发育。在神经层面上， 听力的发展需要听力的适当发展 内耳的感觉受体细胞(内毛细胞和外毛细胞) 它们的神经由传入和传出纤维支配。目前， 分子和形态过程导致了 人们对这种神经系统的分化知之甚少。因此， 我们建议启动一系列调查，以开始 了解这些流程。 这项研究的目的是理解分子和 延髓内传出神经的形态发育 小鼠的科尔蒂器官。最近的研究表明， 传出系统可由离散的神经元亚单位组成 根据继承人的生化特征在功能上有所不同。 因此，我们建议使用生化、组织化学和 免疫细胞化学技术研究血管内皮生长因子的表达 乙酰胆碱代谢酶-胆碱乙酰转移酶 (ChAT)、AChE和AChE的分子形式-以及 神经肽类神经递质蛋氨酸-脑啡肽和 降钙素基因相关肽与超微结构的关系 耳蜗内传出纤维的发育。我们的数据显示 传出纤维的生化表达先于它们的 形态分化。因此，我们希望这些技术 为早期检测传出细胞提供灵敏的探针。 研究纤维的生长和分化模式。 此外，我们关于细菌分子多样性的证据 沿耳蜗尖的胆碱能系统提示有功能 它们的突触结构的不同。这些数据强调了对 选择性免疫学可视化以区分分子 神经性子系统。我们希望通过克隆的方式 针对不同AChE分子形式(球状6/4S、10S)的抗体 和不对称16S)，我们将能够确定 不同子系统的发育时间和地形 在传出系统的形态分化过程中。

Fundamental to the acquisition of communication skills is the normal development of hearing. At the neural level, the development of hearing requires the proper development of the sensory receptor cells in the inner ear (inner and outer hair cells) and their innervation by afferent and efferent fibers. Presently, the molecular and morphological processes that lead to the differentiation of this neural system are poorly understood. Thus, we propose to initiate a series of investigation to begin to understand those processes. The goal of this research is to comprehend the molecular and morphological development of the efferent innervation in the organ of Corti of the mouse. Recent studies suggest that the efferent system may consist of discrete neuronal subunits that differ functionally depending on heir biochemical characteristics. We propose, therefore, to use biochemical, histochemical and immunocytochemical techniques to study the expression of the enzymes of acetylcholine metabolism - choline acetyltransferase (ChAT), AchE and the molecular forms of AChE - and the neuropeptide neurotransmitters methionine-enkepalin and calcitonin gene-related pepteide in relation to the ultrastructural development of efferent fibers in the cochlea. Our data suggests that biochemical expression of the efferent fibers preceed their morphological differentiation. Thus, we expect these techniques to provide a sensitive probe for the early detection of efferent fibers to study the patterns of their growth and differentiation. Futhermore, our evidence of the molecular diversity of the cholinergic system along the cochlear spire suggest functional differences in their synaptic apparatus. The data stress a need for selective immunological visualization to distinguish the molecular neuronal subsystems. We expect that by the means of monoclonal antibodies to different AChE molecular forms (globular 6/4S, 10S and asymmetric 16S) we will be able to determine the developmental time and the topography of different subsystems during the morphological differentiation of the efferent system.