TRANSITIONS AND TRANSPORT IN GASTRIC MUCOSA

胃粘膜的转变和运输

• 批准号: 3226425
• 负责人: TERRY E MACHEN
• 金额: $ 19.82万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-04-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3226425
• 关键词: acid base balance; acidity /alkalinity; adenosinetriphosphatase; antihistamines; bicarbonates; calcium; carbachol; chlorine; cyclic AMP; exocytosis; fluorescence spectrometry; fluorescent dye /probe; gastric mucosa; gastrointestinal absorption /transport; histamine; homeostasis; hydrogen; image processing; ion transport; ionophores; laboratory rabbit; membrane permeability; membrane potentials; membrane transport proteins; microspectrophotometry; muscarinic receptor; potassium; protein kinase C; second messengers; secretion; sodium

The major goal of this work is to determine how the acid-secreting parietal cells of the rabbit stomach maintain ionic homeostasis during large changes in their secretory activity. Microspectrofluorimetry and digital image processing of pH-, Na- Ca-, Cl- and K-sensitive fluorescent dyes will be used to determine how the ion exchangers (Na/H, Cl/HCO3, and Na/Ca), cotransporter (Na/HCO3), ATP-ases (Na/K and H/K), and Ca "channels" are controlled by the H2 agonist histamine and muscarinic agonist carbachol. The roles which different second messengers like Ca, pH, CAMP and protein kinase C may play in stimulus secretion coupling will also be investigated, as will the roles which Na/H and Cl/HC03 exchange might play in maintenance of the "barrier" function of the enzyme-secreting chief cells and mucus-secreting surface epithelial cells. These data will be of importance in knowing how stomach cells operate in healthy and diseased states. Also, since the membrane transport processes and stimulus-secretion coupling mechanisms are present in most secretory cells, the work should have quite broad relevance.

这项工作的主要目标是确定酸分泌如何 兔胃壁细胞在消化过程中维持离子稳态 他们的分泌活动发生巨大变化。显微分光荧光测定法和 pH、Na、Ca、Cl 和 K 敏感荧光的数字图像处理 染料将用于确定离子交换剂（Na/H、Cl/HCO3 和 Na/Ca)、协同转运蛋白 (Na/HCO3)、ATP 酶（Na/K 和 H/K）和 Ca “通道”由 H2 激动剂组胺和毒蕈碱控制 激动剂卡巴胆碱。不同的第二信使如Ca所扮演的角色， pH、CAMP 和蛋白激酶 C 可能在刺激分泌耦合中发挥作用 也将研究 Na/H 和 Cl/HC03 交换的作用 可能会起到维护“屏障”功能的作用 分泌酶的主细胞和分泌粘液的表面上皮细胞。 这些数据对于了解胃细胞如何运作非常重要 健康和患病状态。此外，由于膜运输过程 大多数分泌细胞都存在刺激-分泌耦合机制 细胞，这项工作应该具有相当广泛的相关性。