CARBOHYDRATE AND LIPID METABOLIC PATHWAYS

碳水化合物和脂质代谢途径

• 批准号: 3225231
• 负责人: BERNARD R LANDAU
• 金额: $ 25.3万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 1988-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3225231
• 关键词: NAD(H) phosphate; adenylate cyclase; beta hydroxybutyrate; brain metabolism; carbohydrate metabolism; fatty acid metabolism; fructose; glucagon; gluconeogenesis; hormone regulation /control mechanism; human tissue; hypothalamus; insulin; ketone body; kidney cell; kidney metabolism; lipid metabolism; liver cells; liver metabolism; muscle cells; muscle metabolism; neuroendocrine system; nutrition related tag; oxidation; palmitates; perfusion; placenta; radiotracer; seminal vesicles; sorbitol; split brain; starvation; stearates; steroid biosynthesis; tritium

Methods will be developed and applied to the quantitation of pathways of carbohydrate and lipid metabolism. Using a split monkey brain preparation, an effect of insulin on glucose metabolism by the hypothalamus will be sought. From the fate of C14 of specifically C14-labeled fatty acids, the extent that alpha- and omega- oxidation contribute to fatty acid metabolism will be determined in muscle, liver, and brain during starvation and diabetes mellitus. The fate of the carbons of ketone bodies in these tissues will also be assessed including their possible contribution of intermediates for gluconeogenesis. Yields of C14O2 from specifically labeled fatty acids will be used as a measure of formation of free intermediates in fatty acid oxidation. The source of fructose in placenta will be measured by tracing its formation from C14- and H3-labeled glucose. For this the stereospecificity of the enzymes of the polyol pathway in regard to reduced nucleotide formation will be determined.

方法将被开发和应用于定量的途径， 碳水化合物和脂质代谢。 使用分离的猴脑制备物， 将寻求胰岛素对下丘脑葡萄糖代谢的影响。 从C14标记的脂肪酸的C14的命运来看， α-和ω-氧化有助于脂肪酸代谢， 在饥饿和糖尿病期间的肌肉、肝脏和大脑中测定。 还将评估这些组织中酮体碳的去向 包括它们可能对植物异生作用的中间体的贡献。 将使用来自特定标记的脂肪酸的C14 O2的产率作为测量 在脂肪酸氧化中形成游离中间体。 的来源 胎盘中的果糖将通过追踪其从C14-和 H3标记葡萄糖。 为此，多元醇的酶的立体特异性 将确定关于减少的核苷酸形成的途径。