HUMAN ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
HUMAN ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
批准号:
3229056
负责人:
G Vann Bennett
金额:
$19.95万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1987
资助国家:
美国
项目状态:
已结题
起止时间:
1987-09-01 至 1991-03-31
关键词:
Plasmodium falciparum ankyrins band 3 protein binding proteins calcium calmodulin clathrin cow crosslink cytoskeleton electron microscopy erythrocyte membrane erythroleukemia gel electrophoresis human tissue laboratory rabbit malaria membrane proteins phosphorylation protein structure radioimmunoassay radiotracer spectrin transferrin transferrin receptor virulence
中文摘要
该提案的目标是 a) 更详细地阐明
人红细胞膜蛋白质的分子间缔合
骨架; b) 确定膜蛋白在红细胞生成过程中如何组装
发展; c) 使用红细胞膜作为实验
用于理解膜结构和功能的可访问模型系统
在更复杂的细胞中。 另一个令人感兴趣的新领域是
恶性疟原虫侵袭人红细胞的机制
寄生虫。 作为一般方法,这些研究将通过
已纯化和表征的膜蛋白,以及
这些蛋白质之间的关联将被定量评估。
具体项目包括以下几个方面: 1) 结合的研究
红细胞锚蛋白和锚蛋白的蛋白水解片段在溶液中
确定的血影蛋白寡聚状态和条带的细胞质结构域
3. 2) 对新发现的钙调蛋白结合蛋白的研究
红细胞膜骨架的组成部分,其目标是确定
它的蛋白质关联,以及钙对这些关联的调节
和钙调蛋白。 3) 将 RBC 作为研究模型系统进行评估
受体介导的内吞作用中的蛋白质关联,使用网格蛋白
最近已从红细胞胞浆中纯化出转铁蛋白受体
胎盘。 4) 迄今为止尚未表征的红细胞蛋白的研究
包括纯化、最近邻分析和异构体搜索
在其他组织中。 候选者将是带 4.2,一种膜骨架形式
带 7 和最近发现的钙调蛋白结合蛋白。 5) 组装
红白血病分化过程中红细胞膜骨架的变化
细胞。 6) 人红细胞侵袭机制的研究
恶性疟原虫裂殖子,重点是裂殖子的纯化
与带 3 相互作用的蛋白质。
英文摘要
The goals of this proposal are to a) elucidate in further detail the
intermolecular associations of proteins of the human erythrocyte membrane
skeleton; b) determine how membrane proteins assemble during erythroid
development; c) use the erythrocyte membrane as an experimentally
accessible model system for understanding membrane structure and function
in more complex cells. An additional, new area of interest is the
mechanism of invasion of human erythrocytes by P. falciparum malarial
parasites. As a general approach, these studies will be performed with
membrane proteins that have been purified and characterized, and
associations between these proteins will be evaluated quantitatively.
Specific projects are in the following areas: 1) Studies of binding of
erythrocyte anykrin and proteolytic fragments of ankyrin in solution to
defined oligomeric states of spectrin and the cytoplasmic domain of band
3. 2) Studies of a newly discovered calmodulin-binding protein that is a
component of the erythrocyte membrane skeleton with goals of determining
its protein associations, and regulation of these associations by calcium
and calmodulin. 3) Evaluation of the RBC as a model system to study
protein associations in receptor-mediated endocytosis, using clathrin that
has recently been purified from RBC cytosol, and transferrin receptor from
placenta. 4) Studies of heretofore uncharacterized erythrocyte proteins
including purification, nearest neighbor analysis and search for isoforms
in other tissues. Candidates will be band 4.2, a membrane skeletal form of
band 7 and a recently discovered calmodulin-binding protein. 5) Assembly
of an erythroid membrane skeleton during differentiation of erythroleukemia
cells. 6) Studies of the mechanisms of invasion of human erythrocytes by
merozoites of P. falciparum, with emphasis on purification of merozoite
proteins that interact with band 3.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Deciphering high function autism using mice with human de novo ANK2 mutations
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批准号:9429362
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项目类别:
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资助金额:$23.88万
-
财政年份:2017
-
负责人:G Vann Bennett
-
依托单位:
ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:2138239
-
项目类别:
-
资助金额:$5.34万
-
财政年份:1987
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负责人:G Vann Bennett
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依托单位:
ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:2391339
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项目类别:
-
资助金额:$24.44万
-
财政年份:1987
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负责人:G Vann Bennett
-
依托单位:
ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:2684112
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项目类别:
-
资助金额:$25.42万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
HUMAN ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:3229055
-
项目类别:
-
资助金额:$13.8万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:2138240
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项目类别:
-
资助金额:$20.63万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
GORDON CONFERENCE ON THE RED CELL
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批准号:3435624
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项目类别:
-
资助金额:$1.0万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
HUMAN ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:3229054
-
项目类别:
-
资助金额:$7.02万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:2138237
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项目类别:
-
资助金额:$20.12万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
HUMAN ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:3483522
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项目类别:
-
资助金额:$18.75万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
HUMAN ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:3483523
-
项目类别:
-
资助金额:$19.34万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
HUMAN ERYTHROCYTE MEMBRANE CYTOSKELTON ASSOCIATIONS
-
批准号:3229057
-
项目类别:
-
资助金额:$6.33万
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财政年份:1987
-
负责人:G Vann Bennett
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依托单位:
PROTEIN ASSOCIATIONS OF BRAIN SPECTRIN AND ANKYRIN
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批准号:3284314
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项目类别:
-
资助金额:$7.87万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:2138238
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项目类别:
-
资助金额:$21.29万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
PROTEIN ASSOCIATIONS OF BRAIN SPECTRIN AND ANKYRIN
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批准号:3284313
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项目类别:
-
资助金额:$6.23万
-
财政年份:1987
-
负责人:G Vann Bennett
-
依托单位:
HUMAN ERYTHROCYTE MEMBRANE CYTOSKELETON ASSOCIATIONS
-
批准号:3483521
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项目类别:
-
资助金额:$17.99万
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财政年份:1987
-
负责人:G Vann Bennett
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依托单位:
PROTEIN ASSOCIATIONS OF BRAIN SPECTRIN AND ANKYRIN
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批准号:3284311
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项目类别:
-
资助金额:$13.19万
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财政年份:1984
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负责人:G Vann Bennett
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依托单位:
PROTEIN ASSOCIATIONS OF BRAIN SPECTRIN AND ANKYRIN
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批准号:3284312
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项目类别:
-
资助金额:$3.22万
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财政年份:1984
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负责人:G Vann Bennett
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依托单位:
PROTEIN ASSOCIATIONS OF BRAIN SPECTRIN AND ANKYRIN
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批准号:3284310
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项目类别:
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资助金额:$12.7万
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财政年份:1984
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负责人:G Vann Bennett
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依托单位:
INTERACTION OF ERYTHROCYTE MEMBRANE PROTEINS
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批准号:3071095
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项目类别:
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资助金额:$5.36万
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财政年份:1981
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负责人:G Vann Bennett
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依托单位:
海外基金