CELLULAR PD AND ION ACTIVITIES IN RENAL PROXIMAL TUBULES

肾近端小管中的细胞 PD 和离子活性

• 批准号: 3229596
• 负责人: BRUCE A BIAGI
• 金额: $ 6.15万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-04-01 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3229596
• 关键词: acidity /alkalinity; adenosinetriphosphatase; anions; bicarbonates; cations; homeostasis; intracellular; kidney metabolism; membrane permeability; membrane potentials; microelectrodes; renal cortex; renal tubular transport

The principal aims of this project are to examine in greater detail the highly interdependent relationships between those factors which regulate intracellular sodium activity and net sodium transport and the transport systems for hydrogen and potassium ions. Isolated perfused proximal convoluted and proximal straight tubule segments from the superficial cortex will be compared with regard to transport and cell regulatory functions. The specific aims of the proposed experiments are to: 1) examine the effect of extracellular and intracellular pH on anion and cation perm-selectivity of the luminal and basolateral membranes 2) investigate the underlying mechanisms which result in the difference in apparent bicarbonate conductance of the basolateral membrane between the proximal convoluted and proximal straight tubule 3) examine the phenomenon of electrical potential oscillations in convoluted tubules with respect to the interaction between transport and cellular regulation of pH and 4) investigate the regulation of cellular potassium with particular emphasis on the luminal membrane. Measurements will be made with conventional microelectrodes and with double barreled ion selective electrodes for sodium, potassium, chloride, and hydrogen. The long term goals are to elucidate the basic mechanisms by which cellular ion exchanges and renal transport processes maintain homeostatic control of electrolyte and water balance of the body fluids.

该项目的主要目的是更详细地研究 调节因素之间高度相互依存的关系 细胞内钠活性和净钠转运及转运 氢离子和钾离子系统。 近端隔离灌注 来自浅表的回曲管段和近端直管段 将在运输和细胞调节方面比较皮质 功能。 拟议实验的具体目标是：1） 检查细胞外和细胞内 pH 对阴离子和 管腔膜和基底外侧膜的阳离子渗透选择性 2) 调查导致差异的根本机制 基底外侧膜的表观碳酸氢盐电导 近曲小管和近曲小管 3) 检查现象 曲管中的电势振荡相对于 运输和细胞 pH 调节之间的相互作用 4) 特别强调研究细胞钾的调节 在管腔膜上。 测量将采用常规方法进行 微电极和双筒离子选择性电极 钠、钾、氯和氢。 长期目标是 阐明细胞离子交换和肾功能的基本机制 运输过程维持电解质和水的稳态控制 体液的平衡。