DEVELOPMENT OF RENAL AMINO ACID TRANSPORT

肾脏氨基酸运输的发展

• 批准号: 3233888
• 负责人: MARVIN S MEDOW
• 金额: $ 7.16万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-09-30 至 1987-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3233888
• 关键词: aminoacid transport; growth /development; renal tubular transport

The diminished renal tubule absorptive capacity which is a hallmark of the immature kidney results in the "physiological aminoaciduria" observed in the early postnatal period of rat, dog and man. Adult patterns of renal amino acid reabsorption are gradually achieved as the neonate matures. Although the mechanisms which result in transport maturation remain to be identified, it is possible that changes may occur with age which influence the function of the carrier(s) responsible for the majority of renal amino acid transport. This may be the result of alterations of cellular metabolism to which membrane function is coupled or of developmental variations in membrane structure or composition. Transport maturation is paralleled by, and may in part be due to, significant renal developmental morphological changes which result in increased surface area of both the brushborder and basolateral membranes across which renal solute transport occurs. Altered patterns of amino acid transport have been shown in vitro in renal cortical slices, isolated renal proximal tubules and renal brushborder membranes, comparing newborn to adult. To understand the nature of these age related alterations, solute transport by renal brushborder and basolateral vesicles from animals of various ages will be studied, and the influence of ATP, NAD, NADH and cAMP, known modulators of renal solute transport, will be examined. Physical-chemical characteristics of these membranes will be established by determining membrane fluidity, lipid composition, and the degree of protein phosphorylation as influenced by ATP and cAMP. Age related differences found in these characteristics may lead to a better understanding of the mechanisms which result in the ontogeny of renal transport process. In addition, knowledge of transport events in the newborn renal tubule cell leading to decreased amino acid reabsorption may also help explain the etiology of inherited transport abnormalities for which there are very few animal models and limited investigative procedures in the affected human.

肾小管吸收能力下降，这是一个标志

项目成果

Ontogeny of basolateral membrane lipid composition and fluidity in small intestine.

小肠基底外侧膜脂质成分和流动性的个体发育。

• DOI: 10.1152/ajpgi.1989.257.1.g138
• 发表时间: 1989
• 期刊: The American journal of physiology
• 作者: Schwarz,SM;Bostwick,HE;Danziger,MD;Newman,LJ;Medow,MS
• 通讯作者: Medow,MS