EFFECTS OF REPRODUCTIVE TOXICANTS ON TESTICULAR CELLS

生殖系统是许多化学物质的目标， 家庭、农业或工业用途。 虽然毒性机制 在大多数情况下是未知的，很明显， 雄性对化学物质敏感，如二溴氯丙烷，烷基 乙二醇醚和某些邻苯二甲酸酯。 机制的研究 生殖毒物的复杂性阻碍了 精子发生的生理和生化过程。 但 睾丸的功能单位是生精小管，它是 相对容易分离和培养其主要细胞类型。 这 将使用一个简化的系统来检查化学品的影响 在仔细控制的组织培养条件下， 本研究的目的是：（1）确定 三组生殖毒物的使用离体睾丸 细胞和亚细胞系统，以及（2）更好地了解代谢 男性生殖系统的一部分 最终，一个在 体外系统，能够评估潜在的生殖毒性， 化学品是可以预见的。

The reproductive system is a target of many chemicals that have wide-spread domestic, agricultural, or industrial use. While the mechanism of toxicity is unknown in most cases, it is apparent that the reproductive system of the male is sensitive to chemicals such as dibromochloropropane, the alkyl glycol ethers, and certain phthalate esters. The study of the mechanisms of reproductive toxicants is hampered by the complexity of the physiological and biochemical processes of spermatogenesis. However, the functional unit of the testes is the seminiferous tubule, and it is relatively easy to isolate and culture its primary cell types. This simplified system will be used to examine the effects of the chemicals listed above under the carefully controlled conditions of tissue culture. In brief, the objectives are: (1) to determine the toxic mechanism of three groups of reproductive toxicants by the use of isolated testicular cell and subcellular systems, and (2) to better understand the metabolism of the male reproductive system. Ultimately, the development of an in vitro system capable of assessing the potential reproductive toxicity of a chemical is envisioned.