CLINICAL LASER SPECTROSCOPY OF THE LENS

这项研究的目的是完善临床 激光光散射光谱技术，以便它可以 眼科医生和眼科研究人员经常使用， 白内障早期检测和定量诊断 形成，并用于开发和评价抗白内障 毒品 完成后，我们将拥有第一个 用于透镜的定量、高分子水平的临床工具， 用于透镜的临床和研究。 激光散射光谱是一种安全、无创、 技术，使灵敏的观察布朗 完整透镜内晶状体蛋白的运动。 从 晶体散射光的时间涨落 蛋白质，可以检测到轻微的变化，如 聚集和合成，发生在细胞质中。 十一 自从该技术首次开发以来已经过去了多年， 在我们实验室的兔子身上。 该技术已经 从那时起，它的临床用途得到了改善， 和独特性作为一个定量和极其敏感的临床 工具已经建立。 （安全性得到NIH、MGH的批准， 美国和日本的福利部）。 然而，其功能 作为常规眼科工具的能力尚未完全 实现了 我们将其不完美的根源合理化， 以下：在所观察的位置内的不可再现性 透镜，其原理复杂，分析困难 数据的解释。 我们现在认为， 激光光谱学和蛋白质物理学的进展 解决方案和凝胶，我们相信我们有一些 这些贡献是巨大的，使我们能够解决这些问题。 困难 因此，我们建议完善临床 技术在其光电和机械结构， 超高浓度高分子溶液科学， 凝胶，细胞质，分析方法，解释， 数据的呈现。

The objective of this proposed research is to perfect the clinical laser light scattering spectroscopy technique so that it can be routinely used by ophthalmologists and eye researchers to provide the early detection and quantitative diagnosis of cataract formation, and for the development and evaluation of anti-cataract drugs. Upon completion, we will have practically the first quantitative, macromolecular-level clinical tool for the lens, both for clinical and researches of the lens. Laser light scattering spectroscopy is a safe, non-invasive technique, that enables sensitive observation of the Brownian motions of crystallin proteins within the intact lens. From the temporal fluctuations of light scattered from the crystallin proteins, it is possible to detect slight alterations, such as aggregation and synthesis, which occur in the cytoplasm. Eleven years has elapsed since the technique was first developed and applied on rabbits in our laboratory. The technique has been improved since then toward a clinical usage, and indeed its safety and uniqueness as a quantitative and extremely sensitive clinical tool have been established. (Safety approved by NIH, MGH in the USA, and Ministry of Welfare in Japan.) However, its functional capacity as a routine ophthalmological tool is not yet fully realized. We rationalize the source of its imperfection as follows: irreproducibility of the observed location within the lens, sophistication in its principle, and difficulty of analysis and interpretation of data. We now believe that the recent advances in laser spectroscopy, and the physics of protein solutions and gels, for which we believe that we have some contribution, are substantial and allow us to resolve these difficulties. We, thereby, propose to perfect the clinical technique in its opto-electronic and mechanical structure, the science of extremely highly concentrated macromolecular solution, gels, and cytoplasm, the method of analysis, and interpretation and presentation of data.