CLINICAL LASER SPECTROSCOPY OF THE LENS

这项研究的目的是为了完善临床 激光光散射光谱技术使其能够 眼科医生和眼科研究人员经常使用，以提供 白内障的早期发现和定量诊断 形成，并用于抗白内障的开发和评估 毒品。建成后，我们实际上将拥有第一个 晶状体的定量、大分子水平的临床工具，两者 用于晶状体的临床和研究。 激光光散射光谱学是一种安全、无创的 一种技术，能够灵敏地观察到布朗 晶体蛋白在完整晶状体内的运动。从 晶体散射光的时间涨落 蛋白质，有可能检测到轻微的变化，如 发生在细胞质中的聚集和合成。十一 自从这项技术被首次开发以来，已经过去了很多年 在我们实验室的兔子身上应用。这项技术已经被 从那时起，它在临床应用方面得到了改进，而且确实是安全的 作为数量和极其敏感的临床的独特性 工具已经建立。(安全性由NIH，MGH在 美国和日本的福利部。)然而，它的功能 作为常规眼科工具的能力还没有完全发挥出来 意识到了。我们将其不完美的来源合理化为 如下：观察到的位置在 透镜，原理复杂，分析困难 和数据的解释。我们现在认为，最近的 激光光谱学和蛋白质物理学的进展 解决方案和凝胶，我们相信我们有一些 贡献是巨大的，使我们能够解决这些问题 困难。因此，我们建议完善临床 它的光电和机械结构中的技术， 研究极高浓度大分子溶液的科学， 凝胶和细胞质，分析和解释的方法，和 数据的呈现。

The objective of this proposed research is to perfect the clinical laser light scattering spectroscopy technique so that it can be routinely used by ophthalmologists and eye researchers to provide the early detection and quantitative diagnosis of cataract formation, and for the development and evaluation of anti-cataract drugs. Upon completion, we will have practically the first quantitative, macromolecular-level clinical tool for the lens, both for clinical and researches of the lens. Laser light scattering spectroscopy is a safe, non-invasive technique, that enables sensitive observation of the Brownian motions of crystallin proteins within the intact lens. From the temporal fluctuations of light scattered from the crystallin proteins, it is possible to detect slight alterations, such as aggregation and synthesis, which occur in the cytoplasm. Eleven years has elapsed since the technique was first developed and applied on rabbits in our laboratory. The technique has been improved since then toward a clinical usage, and indeed its safety and uniqueness as a quantitative and extremely sensitive clinical tool have been established. (Safety approved by NIH, MGH in the USA, and Ministry of Welfare in Japan.) However, its functional capacity as a routine ophthalmological tool is not yet fully realized. We rationalize the source of its imperfection as follows: irreproducibility of the observed location within the lens, sophistication in its principle, and difficulty of analysis and interpretation of data. We now believe that the recent advances in laser spectroscopy, and the physics of protein solutions and gels, for which we believe that we have some contribution, are substantial and allow us to resolve these difficulties. We, thereby, propose to perfect the clinical technique in its opto-electronic and mechanical structure, the science of extremely highly concentrated macromolecular solution, gels, and cytoplasm, the method of analysis, and interpretation and presentation of data.