STUDIES OF O2 ACTIVATION BY MOSSBAUER SPECTROSCOPY

这项研究旨在阐明该基因的结构和功能。 含铁蛋白质。并提出了探索电子化的新思路。 模拟结构特征的合成络合物的结构 在蛋白质中观察到金属中心。实验技术 使用的是~(57)Fe穆斯堡尔谱和电子顺磁 共振(EPR)。 (1)铁氧蛋白甲烷双核簇合物的研究 单加氧酶和核糖核苷酸还原酶将继续存在，重点是 FeIIFeII团簇形式及其中间体的电子结构 氧气反应的结果。 (2)这项研究将得到同核和同核的研究的补充 铁氧蛋白的异核模型络合物。 (3)穆斯堡尔谱研究化合物的结构和化学 嗜热嗜热菌细胞色素Ba3的氧还原位 将会继续。将努力确定电子产品的特征 细胞色素A3/CUB偶联位点的结构及寻找 催化反应的中间体。 (4)最近开始的新型FeIV络合物的研究将继续进行，并 扩大了。 (5)还建议研究含有An-Fe的配合物 或通过桥联配体连接到Fe4S4簇上的Ni位。是这样的 络合物是大肠杆菌亚硫酸盐活性中心的有趣模型 还原酶，或许还有一氧化碳脱氢酶。 (6)CO脱氢酶和异青霉素N合成酶的研究 继续。 (7)最后，我们将继续以整数旋转EPR为目标 进一步改进这项新技术的数量方面。

The research proposed seeks to elucidate the structure and function of iron-containing proteins. It is also proposed to explore the electronic structure of synthetic complexes which mimick structural features of the metal centers observed in the proteins. The experimental techniques employed are 57Fe Mossbauer spectroscopy and Electron Paramagnetic Resonance (EPR). (1) Studies of the binuclear clusters of the iron-oxo proteins methane monooxygenase and ribonucleotide reductase will be continued, with focus on the electronic structure of the FeIIFeII cluster forms and intermediates of the oxygen reactions. (2) This research will be complemented by studies of homonuclear and heteronuclear model complexes of iron-oxo proteins. (3) Mossbauer measurements to explore the structure and chemistry of the dioxygen reduction site of cytochrome ba3 from Thermus thermophilus will be continued. Efforts will be made to characterize the electronic structure of the coupled cytochrome a3/CuB site and to search for intermediates of the catalytic reaction. (4) Recently begun studies of novel FeIV complexes will be continued and expanded. (5) It is also proposed to study complexes which contain either an Fe or a Ni site linked by a bridging ligand to an Fe4S4 cluster. Such complexes are interesting models for the active sites of E. coli sulfite reductase and, perhaps, CO dehydrogenase. (6) Studies of CO dehydrogenase and isopenicillin N synthase will be continued. (7) Finally, we will continue to develop integer spin EPR with the goal of further improving the quantitative aspects of this new technique.