课题基金 / 基金详情

MECHANISMS OF PROTEIN-NUCLEIC ACID INTERACTIONS

MECHANISMS OF PROTEIN-NUCLEIC ACID INTERACTIONS
蛋白质-核酸相互作用的机制
批准号：
3273153
负责人：
SUZANNE BOURGEOIS-COHN
金额：
$20.59万
依托单位：
SALK INSTITUTE FOR BIOLOGICAL STUDIES
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
项目状态：
已结题
起止时间：
1978-09-01 至 1986-08-31
来源：
https://reporter.nih.gov/project-details/3273153
关键词：
Escherichia coli affinity chromatography bacteriophage lambda enzyme induction /repression gel electrophoresis genetic manipulation genetic recombination genetic transcription lac operon microorganism genetics molecular weight mutant nucleic acid sequence plasmids radiotracer ultracentrifugation

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中文摘要
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英文摘要
The overall objective of this project is to gain understanding of the physical-chemical mechanism of the reactions between proteins and nucleic acids. We use, as a model system, the regulatory interactions of the lac operon of E. coli. While the mode of action of lac repressor is fairly well understood, the mechanism by which the cAMP receptor protein, CAP, stimulates transcription is entirely obscure. Our immediate goal is to characterize the specific binding of purified CAP protein to the lac promoter. We will also examine the possible effect of CAP binding on the stability of the repressor-operator complex located approximately 50 base pairs away.
期刊论文(3)
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会议论文
Specific binding of the cAMP receptor protein of Escherichia coli to the lactose operon promoter.
大肠杆菌的 cAMP 受体蛋白与乳糖操纵子启动子的特异性结合。
DOI： 10.1016/s0300-9084(85)80244-3
发表时间： 1985
期刊： Biochimie
影响因子： 3.9
作者： [Koop,AH, Staprans,SI, Bourgeois,S]
通讯作者： Bourgeois,S
Analysis of the cya locus of Escherichia coli.
大肠杆菌 cya 位点分析。
DOI： 10.1016/0378-1119(84)90250-6
发表时间： 1984
期刊： Gene
影响因子： 3.5
作者： [Koop,AH, Hartley,M, Bourgeois,S]
通讯作者： Bourgeois,S
REGULATION OF EXTRACELLULAR MATRIX BIOSYNTHESIS
REGULATION OF EXTRACELLULAR MATRIX BIOSYNTHESIS
REGULATION OF EXTRACELLULAR MATRIX BIOSYNTHESIS
REGULATION OF EXTRACELLULAR MATRIX BIOSYNTHESIS
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