TRANSPORT & ENZYMOLOGY OF SULFUR AND NITROGEN COMPOUNDS
TRANSPORT & ENZYMOLOGY OF SULFUR AND NITROGEN COMPOUNDS
批准号:
3274136
负责人:
IRWIN H SEGEL
金额:
$10.73万
依托单位国家:
美国
项目类别:
财政年份:
1979
资助国家:
美国
项目状态:
已结题
起止时间:
1979-07-01 至 1988-08-31
中文摘要
我们的研究是关于新陈代谢的早期阶段
英文摘要
Our research is concerned with the early steps in the metabolism of
inorganic sulfur and nitrogen compounds. Major emphasis is on ATP
sulfurylase and APS kinase from the filamentous fungus Penicillium
chrysogenum. The fungal ATP sulfurylase has been purified to homogeneity
and partially characterized kinetically, physically, and chemically. Our
current efforts are aimed at (a) elucidating the mechanism of the reaction,
(b) identifying the amino acid residues responsible for the functional
integrity of the enzyme, and (c) exploring the potential interaction of ATP
sulfurylase with APS kinase. Parallel studies are in progress on the ATP
sulfurylase from Penicillium duponti (a thermophile). Our work with the P.
duponti enzyme may shed some light on the factors responsible for the
remarkable heat stability of this enzyme. APS kinase, the second sulfate
activating enzyme has been purified to homogeneity from P. chrysogenum and
is being characterized. Studies on the sulfate reduction pathway of fungi
will be initiated. Other proposed projects include studies on nitrate
reductase and nitrite reductase from P. chrysogenum, with particular
emphasis on the kinetic mechanisms of these enzymes. Some of the methods
that will be employed include (a) analysis of steady-state and reaction
progress kinetics, (b) equilibrium ligand binding with native and
chemically modified enzymes, and (c) chemical modification of specific
amino acid residues.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
TRANSPORT & ENZYMOLOGY OF SULFUR AND NITROGEN COMPOUNDS
-
批准号:3274135
-
项目类别:
-
资助金额:$10.92万
-
财政年份:1979
-
负责人:IRWIN H SEGEL
-
依托单位:
TRANSPORT & ENZYMOLOGY OF SULFUR AND NITROGEN COMPOUNDS
-
批准号:3274137
-
项目类别:
-
资助金额:$10.99万
-
财政年份:1979
-
负责人:IRWIN H SEGEL
-
依托单位:
海外基金