MECHANISM OF AUTOTROPHIC GROWTH WITH CO OR CO2 & H2
MECHANISM OF AUTOTROPHIC GROWTH WITH CO OR CO2 & H2
批准号:
3272655
负责人:
Ganesh K Kumar
金额:
$5.18万
依托单位国家:
美国
项目类别:
财政年份:
1978
资助国家:
美国
项目状态:
已结题
起止时间:
1978-04-01 至 1994-06-30
关键词:
Clostridium Mossbauer spectrometry acetates acetyl coA bioenergetics carbon dioxide carbon monoxide chemical binding circular dichroism coenzyme A electron nuclear double resonance spectroscopy electron spin resonance spectroscopy enzyme mechanism enzyme structure hydrogen methyl group methyltransferase microorganism metabolism molecular cloning nuclear magnetic resonance spectroscopy oxidoreductase protein structure tetrahydrofolates
中文摘要
这些调查有作为最终客观的阐明
英文摘要
These investigations have as the ultimate objective elucidation of the
pathway by which certain bacteria grow with CO or CO2 and H2 as the source
of carbon and energy. This is a pathway of autotrophic growth which
differs from any previously described. It involves reduction of CO2 to
methyltetrahydrofolate, then combination of this methyl group with CO and
CoASH to form acetyl-CoA which is then used as the source of carbon for
anabolic growth. The synthesis of acetyl-CoA from methyltetrahydrofolate,
CO and CoASH is the unique portion of this pathway. Homogeneous proteins
have been isolated from Clostridium thermoaceticum which catalyze this
synthesis. They are CO dehydrogenase methyltransferase, a corrinoid
enzyme, ferredoxin, and disulfide reductase. The CO dehydrogenase is the
master enzyme of this pathway. It forms the Cl intermediate from CO or CO2
that is the precursor of the carbonyl group of acetyl-CoA, is the acceptor
of the methyl from the methyl corrinoid enzyme and through the action of
the CO dehydrogenase reductase combines with CoA. It then catalyzes the
condensation of these three groups to acetyl-CoA. Our main objective is to
elucidate how CO dehydrogenase catalyzes this condensation. We will use
electron spin resonance (ESR), electron-nuclear double resonance,
Mossbauer, and magnetic circular dichroism techniques to characterize the
geometry and redox state of the Ni-Fe-S center of the CO dehydrogenase. We
will investigate the mechanism of transfer of the methyl group from the
methylated corrinoid protein to CO dehydrogenase with formation of an
acetyl intermediate. We hope to detect this methyl-CO dehydrogenase
complex using ESR techniques and chemically identify the amino acid
sequence at the binding site. Likewise, the sequence near the linkage of
CoASH to the enzyme, presumed to be by a disulfide bond, will be
investigated. The monomers of the Alpha3Beta3 enzyme will be isolated,
their metal content determined and genes for these proteins will be cloned
and sequenced. The properties of CO dehydrogenase from certain
methanogenic bacteria will be tested for their ability to synthesize
acetyl-CoA to determine whether they may use a pathway of acetyl-CoA
synthesis similar to that of C. thermoaceticum.
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The primary structure of the subunits of carbon monoxide dehydrogenase/acetyl-CoA synthase from Clostridium thermoaceticum.
热乙酸梭菌一氧化碳脱氢酶/乙酰辅酶A合酶亚基的一级结构。
DOI:
--
发表时间:
1991
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Morton,TA, Runquist,JA, Ragsdale,SW, Shanmugasundaram,T, Wood,HG, Ljungdahl,LG]
通讯作者:
Ljungdahl,LG
Glycine synthase of the purinolytic bacterium, Clostridium acidiurici. Purification of the glycine-CO2 exchange system.
嘌呤分解细菌,酸尿梭菌的甘氨酸合酶。
DOI:
--
发表时间:
1984
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Gariboldi,RT, Drake,HL]
通讯作者:
Drake,HL
Interaction of ferredoxin with carbon monoxide dehydrogenase from Clostridium thermoaceticum.
铁氧还蛋白与来自热乙酸梭菌的一氧化碳脱氢酶的相互作用。
DOI:
--
发表时间:
1992
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Shanmugasundaram,T, Wood,HG]
通讯作者:
Wood,HG
Role of carbon monoxide dehydrogenase in acetate synthesis by the acetogenic bacterium, Acetobacterium woodii.
一氧化碳脱氢酶在产乙酸细菌伍氏醋杆菌合成乙酸盐中的作用。
DOI:
--
发表时间:
1988
期刊:
BioFactors (Oxford, England)
影响因子:
--
作者:
[Shanmugasundaram,T, Ragsdale,SW, Wood,HG]
通讯作者:
Wood,HG
The autotrophic pathway of acetogenic bacteria. Role of CO dehydrogenase disulfide reductase.
产乙酸细菌的自养途径。
DOI:
--
发表时间:
1986
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Pezacka,E, Wood,HG]
通讯作者:
Wood,HG
共 10 条
Neurotransmitter Metabolism in Intermittent Hypoxia
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批准号:7464291
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项目类别:
-
资助金额:$38.38万
-
财政年份:2008
-
负责人:Ganesh K Kumar
-
依托单位:
Neurotransmitter Metabolism in Intermittent Hypoxia
-
批准号:8050610
-
项目类别:
-
资助金额:$38.38万
-
财政年份:2008
-
负责人:Ganesh K Kumar
-
依托单位:
Neurotransmitter Metabolism in Intermittent Hypoxia
-
批准号:7806365
-
项目类别:
-
资助金额:$38.38万
-
财政年份:2008
-
负责人:Ganesh K Kumar
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依托单位:
Neurotransmitter Metabolism in Intermittent Hypoxia
-
批准号:7587950
-
项目类别:
-
资助金额:$38.38万
-
财政年份:2008
-
负责人:Ganesh K Kumar
-
依托单位:
Neurotransmitter metabolism in intermittent hypoxia
-
批准号:6580880
-
项目类别:
-
资助金额:$24.88万
-
财政年份:2002
-
负责人:Ganesh K Kumar
-
依托单位:
NEUTRAL ENDOPEPTIDASE AND CAROTID BODY FUNCTIONS
-
批准号:2222945
-
项目类别:
-
资助金额:$17.43万
-
财政年份:1995
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负责人:Ganesh K Kumar
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依托单位:
NEUTRAL ENDOPEPTIDASE AND CAROTID BODY FUNCTIONS
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批准号:2222946
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项目类别:
-
资助金额:$18.29万
-
财政年份:1995
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负责人:Ganesh K Kumar
-
依托单位:
NEUROPEPTIDASES AND CAROTID BODY FUNCTIONS
-
批准号:2693324
-
项目类别:
-
资助金额:$20.01万
-
财政年份:1995
-
负责人:Ganesh K Kumar
-
依托单位:
NEUROPEPTIDASES AND CAROTID BODY FUNCTIONS
-
批准号:6183215
-
项目类别:
-
资助金额:$22.29万
-
财政年份:1995
-
负责人:Ganesh K Kumar
-
依托单位:
NEUTRAL ENDOPEPTIDASE AND CAROTID BODY FUNCTIONS
-
批准号:2392674
-
项目类别:
-
资助金额:$19.1万
-
财政年份:1995
-
负责人:Ganesh K Kumar
-
依托单位:
NEUROPEPTIDASES AND CAROTID BODY FUNCTIONS
-
批准号:6030625
-
项目类别:
-
资助金额:$21.64万
-
财政年份:1995
-
负责人:Ganesh K Kumar
-
依托单位:
MECHANISM OF AUTOTROPHIC GROWTH WITH CO OR CO2 AND H2
-
批准号:3272654
-
项目类别:
-
资助金额:$19.86万
-
财政年份:1978
-
负责人:Ganesh K Kumar
-
依托单位:
Neurotransmitter metabolism in intermittent hypoxia
-
批准号:7039126
-
项目类别:
-
资助金额:$29.75万
-
财政年份:--
-
负责人:Ganesh K Kumar
-
依托单位:
海外基金