MECHANISM OF PROTEIN SYNTHESIS INTITIATION

这项研究建议的主要重点是对 蛋白质合成起始和延伸的机制。目前的努力是 重点介绍了每种成分的提纯和表征 这些过程所需的。到目前为止，其中七种蛋白质已经被 纯化和鉴定(EF-α、EF-2、EIF-2、EIF-2A、EIF-4A、EIF-4C、 EIF-4D、eIF-5)。EF-β伽马增量、EIF-31和EIF-4B正在 目前有其特点。有了这些蛋白质，我们将尝试 确定任何其他已被报道的刺激或 模型分析或血红蛋白合成。具体地，EIF-1、Co-EIF-2A(AS 由Gupta和同事描述)，ESP(由Ochoa和同事描述)， 和24,000个道尔顿信使核糖核酸结合蛋白(如Shatkin和 同事)将接受检查。 除了鉴定蛋白质所需的那些蛋白质外 合成，实验正在进行中，以确定生物功能 这些蛋白质，无论是在因子的顺序利用方面还是在 在定义给定因素可能具有的交互作用方面 氨酰-tRNA、三磷酸核苷酸、信使核糖核酸、核糖体或其他因子。 目前，主要的研究领域是信使核糖核酸的识别和结合。 至40s亚基，以及伴随而来的ATP水解性需求。

The major emphasis of this research proposal is an understanding of the mechanism of protein synthesis initiation and elongation. Current efforts are focused upon the purification and characterization of each of the components required for these processes. To date seven of these proteins have been purified and characterized (EF-alpha, EF-2, eIF-2, eIF-2A, eIf-4A, eIF-4C, eIF-4D, eIF-5). EF-beta gamma delta , eIF-31, and eIF-4B are being characterized at present. With these proteins, attempts will be made to identify any additional proteins which have been reported to stimulate either model assays or hemoglobin synthesis. In particular, eIF-1, Co-eIF-2A (as described by Gupta and coworkers), ESP (as described by Ochoa and coworkers), and the 24,000 dalton mRNA binding protein (as described by Shatkin and coworkers) will be examined. In addition to the indentification of those protein required for protein synthesis, experiments are in progress to define the biological function of these proteins, either in terms of the sequential utilization of the factors or in terms of defining the interactions a given factor may have with aminoacyl-tRNA, nucleotide triphosphates, mRNA, ribosomes or another factor. Presently, the major area of examination is the recognition and binding of mRNA to 40S subunits and the concomitant requirement for ATP hydrolysis.