MECHANISM OF PROTEIN SYNTHESIS INTITIATION

蛋白质合成起始机制

• 批准号: 3274247
• 负责人: WILLIAM C. MERRICK
• 金额: $ 18.26万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3274247
• 关键词: RNA methylation; adenosine triphosphate; aminoacid analyzer; density gradient ultracentrifugation; eukaryote; gel electrophoresis; messenger RNA; radiotracer; tritium

The major emphasis of this research proposal is an understanding of the mechanism of protein synthesis initiation and elongation. Current efforts are focused upon the purification and characterization of each of the components required for these processes. To date seven of these proteins have been purified and characterized (EF-alpha, EF-2, eIF-2, eIF-2A, eIf-4A, eIF-4C, eIF-4D, eIF-5). EF-beta gamma delta , eIF-31, and eIF-4B are being characterized at present. With these proteins, attempts will be made to identify any additional proteins which have been reported to stimulate either model assays or hemoglobin synthesis. In particular, eIF-1, Co-eIF-2A (as described by Gupta and coworkers), ESP (as described by Ochoa and coworkers), and the 24,000 dalton mRNA binding protein (as described by Shatkin and coworkers) will be examined. In addition to the indentification of those protein required for protein synthesis, experiments are in progress to define the biological function of these proteins, either in terms of the sequential utilization of the factors or in terms of defining the interactions a given factor may have with aminoacyl-tRNA, nucleotide triphosphates, mRNA, ribosomes or another factor. Presently, the major area of examination is the recognition and binding of mRNA to 40S subunits and the concomitant requirement for ATP hydrolysis.

本研究计划的主要重点是了解