MAMMALIAN CELL SURFACE GENETICS AND BIOCHEMISTRY

我们将继续研究它的结构、功能和动力学 真核细胞的表面膜成分。我们 已经使用细菌和植物毒素作为探针来调查 这些毒素进入和传递到 中国仓鼠卵巢(CHO)细胞胞浆。我们已经雇佣了 这些毒素用于选择在体细胞中有缺陷的突变体 醉酒的过程。未来五年的研究将是 面向(1)膜的脂肪酰化研究 CHO和酵母突变体中的糖蛋白在N- 糖基化；(2)CHO突变体的分离和鉴定 对离子载体莫能菌素和黑曲霉素具抗药性； 白喉缺陷Vero细胞突变体的特性研究 毒素受体；(4)CHO的分离和鉴定 毒素摄取、转运和释放存在缺陷的突变株 进入细胞质，包括条件致死的突变体。 将采用的技术包括分子细胞和体细胞 结构、功能和功能的遗传学和生化分析 参与受体的膜成分的动力学- 介导的毒素内吞作用。这些研究的结果应该是 更好地了解细胞内的蛋白质 运输过程与翻译后修饰的作用 细胞膜糖蛋白在细胞生理学中的作用。

We will continue to study the structure, functions, and dynamics of the surface membrane components of eukaryotic cells. We have used bacterial and plant toxins as probes to investigate the pathways for the entry and delivery of these toxins into the cytosol of Chinese hamster ovary (CHO) cells. We have employed these toxins to select somatic cell mutants defective in the intoxication process. Studies in the next five years will be directed towards (1) studies on the fatty acylation of membrane glycoproteins in CHO and yeast mutants altered in N- glycosylation; (2) isolation and characterization of CHO mutants resistant to ionophores monensin and nigericin; (3) isolation and characterization of Vero cell mutants defective in diphtheria toxin receptor; and (4) isolation and characterization of CHO mutants defective in the uptake, transport and release of toxins into the cytosol, including the conditionally lethal mutants. Techniques to be employed include molecular and somatic cell genetics and biochemical analysis of the structures, functions and dynamics of membrane components involved in the receptor- mediated endocytosis of toxins. Results from these studies should provide a better understanding of the intracellular protein transport process and the role of post-translational modification of membrane glycoproteins in cellular physiology.