MAMMALIAN CELL SURFACE GENETICS AND BIOCHEMISTRY

我们将继续研究其结构、功能和动力学 真核细胞的表面膜成分。 我们 用细菌和植物毒素作为探针来研究 这些毒素进入和输送到 中国仓鼠卵巢（CHO）细胞的胞质溶胶。 我们已经采用 这些毒素，以选择体细胞突变体的缺陷， 中毒过程 未来五年的研究将是 针对（1）膜的脂肪酰化的研究 CHO和酵母突变体中的糖蛋白在N- 糖基化;（2）CHO突变体的分离和表征 抗离子载体莫能菌素和尼日利亚菌素;（3）分离和 白喉缺陷的Vero细胞突变体的表征 毒素受体;（4）CHO的分离和鉴定 毒素吸收、运输和释放缺陷突变体 包括条件致死突变体 所采用的技术包括分子和体细胞 遗传学和生化分析的结构，功能和 参与受体的膜成分的动力学- 介导的毒素内吞作用。 这些研究的结果应该 更好地了解细胞内蛋白质 转运过程和翻译后修饰的作用 细胞生理学中的膜糖蛋白。

We will continue to study the structure, functions, and dynamics of the surface membrane components of eukaryotic cells. We have used bacterial and plant toxins as probes to investigate the pathways for the entry and delivery of these toxins into the cytosol of Chinese hamster ovary (CHO) cells. We have employed these toxins to select somatic cell mutants defective in the intoxication process. Studies in the next five years will be directed towards (1) studies on the fatty acylation of membrane glycoproteins in CHO and yeast mutants altered in N- glycosylation; (2) isolation and characterization of CHO mutants resistant to ionophores monensin and nigericin; (3) isolation and characterization of Vero cell mutants defective in diphtheria toxin receptor; and (4) isolation and characterization of CHO mutants defective in the uptake, transport and release of toxins into the cytosol, including the conditionally lethal mutants. Techniques to be employed include molecular and somatic cell genetics and biochemical analysis of the structures, functions and dynamics of membrane components involved in the receptor- mediated endocytosis of toxins. Results from these studies should provide a better understanding of the intracellular protein transport process and the role of post-translational modification of membrane glycoproteins in cellular physiology.