METALLOENZYMES AND CONNECTIVE TISSUE METABOLISM

这项研究的目的是要探讨其作用机制。 胶原酶的作用，胶原酶的独特能力是催化 生理条件下胶原蛋白的降解。胶原蛋白， 结缔组织的主要结构蛋白，对 被所有已知的消化酶和细胞内酶攻击。因此，行动 胶原酶对所有涉及降解或 结缔组织的重塑。胶原酶，据信是 锌金属酶，将根据不同的来源进行分离 公布的程序和通过亲和层析的方法 发展起来的。所获得的酶将根据以下方面进行表征 它们的同质性、金属含量、氨基酸组成、相对分子质量 和亚基结构。金属原子(S)在催化中的作用将是 通过检测金属取代对酶的影响进行研究 活动。这些酶对天然的底物专一性 胶原蛋白(I-IV型)和一系列合成肽将被 仔细检查了一下。将进行化学改性反应，以 确定必需氨基酸残基。这项研究应该会促进 更好地理解胶原酶在哺乳动物结缔组织中的作用 组织新陈代谢。

The objective of the proposed research is to investigate the mechanism of action of collagenases, which are unique in their ability to catalyze the degradation of collagen under physiological conditions. Collagen, the major structural protein of connective tissue, is highly resistant to attack by all known digestive and intracellular enzymes. Hence, the action of collagenases is essential to all processes involving the degradation or remodeling of connective tissue. Collagenases, which are believed to be zinc metalloenzymes, will be isolated from various sources according to published procedures and by affinity chromatographic methods to be developed. The enzymes obtained will be characterized with respect to their homogeneity, metal content, amino acid composition, molecular weight and subunit structure. The role of the metal atom(s) in catalysis will be investigated by examining the effect of metal substitutions on enzymatic activity. The substrate specificities of these enzymes toward native collagen (Types I-IV) and towards a series of synthetic peptides will be examined in detail. Chemical modification reactions will be carried out to identify essential amino acid residues. This research should promote a greater understanding of the role of collagenases in mammalian connective tissue metabolism.