STRUCTURE-FUNCTION OF GLYCOSYLTRANSFERASES AND LECTINS

糖基转移酶和凝集素的结构-功能

• 批准号: 3273295
• 负责人: ROBERT L HILL
• 金额: $ 35.44万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-01 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3273295
• 关键词: affinity chromatography; antibody; carbohydrate biosynthesis; carbohydrate structure; chemical structure function; enzyme complex; enzyme structure; fucose; glycolipids; glycosyltransferase; growth /development; high performance liquid chromatography; isomerase; liver cells; liver metabolism; molecular cloning; oligosaccharides; other phosphotransferase; radiotracer; tissue /cell culture; transferase

The molecular basis of the actions of three groups of proteins that are involved in aspects of complex carbohydrate function will be examined. 1) GLYCOSYLTRRANSFERASES that catalyze the synthesis of oligosaccharides in glycoconjugates will be examined in several ways. A) Selected transferases will be isolated and their properties characterized. B) Antibodies to selected transferases will be used to localize the enzymes intracellularly, to examine whether they exist as multienzyme complexes and as probes for the structure of the transferase. C) The biosynthetic roles of selected transferases will be sought. D) The possible role of covalently bound lipids in the transferases and the effect of biological lipids on the properties of selected transferases will be examined. E) Genes for selected transferases will be cloned to obtain sufficient enzyme for structure/function studies. 2) LECTINS. Vertebrate liver lectins, primarily the fucose-specific lectin will be examined from several aspects. A) Antibodies against the lectins will be used to define the subcellular localization of lectins. B) Naturally occurring ligands for the lectins will be sought. C) Structural studies on the fucose lectin will be performed. D) The possible roles of lectins during embryonic and fetal development will be sought. 3) ENZYMES FOR NUCLEOTIDESUGAR INTERCONVERSION. Key enzymes that act in nucleotide sugar biosynthesis will be isolated and characterized with emphasis on understanding the molecular mechanisms that act in regulation of nucleotidesugar biosynthesis.

三组蛋白质作用的分子基础是 涉及复杂碳水化合物功能的方面将进行考察。1) 催化低聚糖合成的糖链转移酶 糖偶联物将以几种方式进行检查。A)选定的转移酶 将被分离出来，并对其性质进行表征。B)抗病毒抗体 选定的转移酶将用于在细胞内定位酶， 以检查它们是否以多酶复合体和探针的形式存在 转移酶的结构。C)选定的生物合成作用 将寻求转移酶。D)共价结合的可能作用 转移酶中的脂类及生物脂类对转移酶的影响 将检查选定的转移酶的性质。E)基因 选定的转移酶将被克隆，以获得足够的酶 结构/功能研究。2)凝集素。脊椎动物肝脏凝集素， 首先，岩藻糖特有的凝集素将从几个 方面。A)将使用针对凝集素的抗体来定义 凝集素的亚细胞定位。B)天然存在的配体 凝集素将被寻找。C)岩藻糖凝集素的结构研究 将会被执行。D)凝集素在胚胎和胚胎发育过程中的可能作用 将寻求胎儿发育。3)核糖核酶 相互转换。核苷酸糖生物合成中的关键酶 将是孤立的，其特点是强调理解 调节核苷酸糖生物合成的分子机制。