SURFACE FUNCTIONS DURING MITOSIS

现在已知有丝分裂过程中膜功能的深刻变化 发生。内吞作用实际上停止了。ConA受体复合体出现 固定不动，不打补丁或形成帽子，直到它们迅速移动到 卵裂(柏林等人，Cell，1978，15：327；J.Cell Biol.，1980， 85：660)。提出了一系列的实验：1)测定 配基-受体进入卵裂沟和卵裂沟的机制 特别是为了测试它根据一种新模型发生的可能性 2)分析C3b结合改变的机制 (补体)包被颗粒到有丝分裂细胞；3)测定 膜功能与染色体周期和 胞质分裂；4)确定是否有额外的膜功能改变 在有丝分裂过程中；以及5)检查膜变化在过程中的作用 有丝分裂为间期膜的后续组织 细胞。实验方法依赖于单电池技术和 发展一种方法来精确识别疾病的各个阶段 有丝分裂、有丝分裂进程的时间分析和胞饮作用。 横向扩散将通过荧光恢复来测量，在 照片漂白。分析波浪运动的一种新方法是 提出采用量化的视频图像处理方法。电生理学 有丝分裂心肌细胞的记录将使用标准 技巧。巨噬细胞系J774将用于移动 使用荧光凝集素和抗C3b受体的F(Ab)2‘的研究。 鸡胚胎心肌细胞将用于电生理 节拍频率、动作电位和反应的研究 β肾上腺素能药物。一种荧光β-肾上腺素能类似物的应用 (9AAP)将被重新检查，并确定其分布和结合。一个 荧光α-MSH类似物将被用来研究结合迁移率 和α-MSH受体的分布。我们的总体目标是获得 关于配体-受体运动和控制机制的独特信息 内吞作用。我们希望了解处于有丝分裂状态的细胞是否在 它们对外界信号的反应中的膜水平，以及是否 有丝分裂中的细胞膜变化是细胞进一步分化的关键 细胞。这一结果应该对所有生物系统都有意义 哪些细胞分裂或表面功能是重要的。

Profound changes in membrane function during mitosis are now known to occur. Endocytosis virtually stops. Con A-receptor complexes appear immobile, without patching or cap formation, until they move rapidly into the cleavage (Berlin et al., Cell, 1978, 15:327; J. Cell Biol., 1980, 85:660). A series of experiments is proposed: 1) to determine the mechanism of ligant-receptor movement into the cleavage furrow and in particular to test the possibility that it occurs according to a new model of wave motion; 2) to analyze the mechanism of altered binding of C3b (complement) coated particles to mitotic cells; 3) to determine the coordination of membrane function with the chromosome cycle and cytokinesis; 4) to determine if additional membrane functions are altered during mitosis; and 5) to examine the role of membrane changes during mitosis for the subsequent organization of the membrane of interphase cells. The experimental approach relies on single cell techniques and the development of a methodology for precise identification of stages of mitosis, temporal analysis of the progression of mitosis, and pinocytosis. Lateral diffusion will be measured by fluorescence recovery after photo-bleaching. A new approach for the analysis of wave motion is proposed using quantitative video image processing. Electrophysiological recordings from mitotic myocardial cells will be made using standard techniques. The macrophage cell line J774 will be employed for mobility studies using fluorescent lectins and the F(ab)2' fo an anti-C3b receptor. Chick embryonic myocardial cells will be used for electrophysiological studies of beat frequency, action potential and responses to beta-adrenergic drugs. The use of a fluorescent beta-adrenergic analogue (9AAP) will be reexamined and its distribution and binding determined. A fluorescent alpha-MSH analogue will be used to study the binding mobility and distribution of the alpha-MSH receptor. We aim overall to obtain unique information on the mechanism of ligand-receptor movement and control of endocytosis. We hope to learn whether cells in mitosis are altered at the membrane level in their response to external signals, and whether the membrane changes in mitosis are critical in the further differentiation of cells. The results should have significance for all biological systems in which cell division or surface functions are important.