MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
批准号:
3276314
负责人:
DANIEL A. GOODENOUGH
金额:
$13.44万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1981
资助国家:
美国
项目状态:
已结题
起止时间:
1981-04-01 至 1989-03-31
关键词:
antibody formation antibody specificity bile ducts brush border membrane density gradient ultracentrifugation electron microscopy electrophysiology freeze etching gastrointestinal epithelium gel electrophoresis immunochemistry immunoelectron microscopy immunofluorescence technique immunoprecipitation intercellular connection intestines liver membrane permeability molecular biology surface antigens tissue /cell culture
中文摘要
分隔生理区室的生物上皮细胞充当
离子跨上皮通道的渗透性屏障,
分子。 细胞之间的空间,称为细胞旁通路,
被一个垫圈状的细胞间连接封闭起来,
(ZO)。 ZO是一系列细胞间连接的最顶端成员,
连接,统称为连接复合体。 新的制备
从啮齿动物肝脏分离的连接复合物制备,
通过形态学技术证明含有结构成分
的ZO。 这些复合物的聚丙烯酰胺凝胶分析显示,
与整个多肽相比,
肝细胞质膜 使用单克隆和多克隆抗体
方法,在本申请中提出生成特定的
这些连接复合物制剂的抗血清,并鉴定
这些抗血清的特异性。 使用
免疫电子显微镜,抗原的解剖位置
被这些抗血清识别的抗体将定位在连接复合物内,
其目的是特异性地鉴定
ZO 一旦确定,将研究ZO的生物起源和组装
在整个合成后使用抗原的免疫沉淀
肝脏或粗微粒体富集的polyA mRNA组分。 但愿,
这些抗血清在生物运输的研究中也将证明是有价值的
在运输上皮细胞的培养中。
英文摘要
Biological epithelia which separate physiological compartments act as
permeability barriers to the transepithelial passage of ions and
molecules. The spaces between the cells, called the paracellular pathway,
are sealed by a gasket-like intercellular junction, the zonula occludens
(ZO). The ZO is the apical-most member of a series of intercellular
junctions, collectively termed the junctional complex. A novel preparation
of isolated junctional complexes from rodent liver has prepared, which is
demonstrated by morphological techniques to contain structural components
of the ZO. Polyacrylamide gel analysis of these complexes reveals a
greatly simplified profile of polypeptides, compared to the whole
hepatocyte plasma membrane. Using monoclonal and polyclonal antibody
approaches, it is proposed in this application to generate specific
antisera to these junctional complex preparations and to identify the
specificity of these antisera by immunoreplica techniques. Using
immunoelectron microscopy, the anatomical locations of the antigens
recognized by these antisera will be mapped within the junctional complex,
with the goal of specifically identifying the molecular components of the
ZO. Once identified, the biogenesis and assembly of the ZO will be studied
using immune precipitation of antigens following synthesis in either whole
liver or rough microsome enriched fractions of polyA mRNA. Hopefully,
these antisera will also prove valuable in studies of biological transport
in cultures of transporting epithelia.
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MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
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批准号:3276313
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项目类别:
-
资助金额:$13.13万
-
财政年份:1981
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
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批准号:3276318
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项目类别:
-
资助金额:$14.41万
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财政年份:1981
-
负责人:DANIEL A. GOODENOUGH
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依托单位:
MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
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批准号:3276311
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项目类别:
-
资助金额:$11.84万
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财政年份:1981
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
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批准号:3276317
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项目类别:
-
资助金额:$13.05万
-
财政年份:1981
-
负责人:DANIEL A. GOODENOUGH
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依托单位:
MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
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批准号:3276319
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项目类别:
-
资助金额:$14.99万
-
财政年份:1981
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
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批准号:3276316
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项目类别:
-
资助金额:$13.31万
-
财政年份:1981
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
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批准号:3276315
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项目类别:
-
资助金额:$14.52万
-
财政年份:1981
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
MOLECULAR BIOLOGY OF ZONULAE OCCLUDENTES
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批准号:2175321
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项目类别:
-
资助金额:$15.17万
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财政年份:1981
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负责人:DANIEL A. GOODENOUGH
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依托单位:
LENS METABOLIC COOPERATION, GAP JUNCTIONS AND CATARACT
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批准号:2158430
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项目类别:
-
资助金额:$23.57万
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财政年份:1978
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负责人:DANIEL A. GOODENOUGH
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依托单位:
Connexin Molecular Biology in the Lens and Ciliary Epithelium
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批准号:7389484
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项目类别:
-
资助金额:$37.26万
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财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
LENS METABOLIC COOPERATION GAP JUNCTIONS AND CATARACT
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批准号:2378035
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项目类别:
-
资助金额:$35.26万
-
财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
LENS METABOLIC COOPERATION GAP JUNCTIONS AND CATARACT
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批准号:2668366
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项目类别:
-
资助金额:$36.39万
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财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
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依托单位:
LENS METABOLIC COOPERATION, GAP JUNCTIONS & CATARACT
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批准号:3256773
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项目类别:
-
资助金额:$23.32万
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财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
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依托单位:
LENS METABOLIC COOPERATION, GAP JUNCTIONS, & CATARACT
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批准号:3256778
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项目类别:
-
资助金额:$21.13万
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财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
LENS METABOLIC COOPERATION, GAP JUNCTIONS & CATARACT
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批准号:3256772
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项目类别:
-
资助金额:$19.27万
-
财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
LENS METABOLIC COOPERATION GAP JUNCTIONS AND CATARACT
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批准号:6806829
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项目类别:
-
资助金额:$13.56万
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财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
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依托单位:
LENS METABOLIC COOPERATION GAP JUNCTIONS AND CATARACT
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批准号:6635552
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项目类别:
-
资助金额:$43.0万
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财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
-
依托单位:
LENS METABOLIC COOPERATION, GAP JUNCTIONS AND CATARACT
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批准号:2158431
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项目类别:
-
资助金额:$23.97万
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财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
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依托单位:
LENS METABOLIC COOPERATION GAP JUNCTIONS AND CATARACT
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批准号:6867308
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项目类别:
-
资助金额:$43.0万
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财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
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依托单位:
LENS METABOLIC COOPERATION GAP JUNCTIONS AND CATARACT
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批准号:6164642
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项目类别:
-
资助金额:$38.78万
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财政年份:1978
-
负责人:DANIEL A. GOODENOUGH
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依托单位:
海外基金