C-GLUCURONIDATION--NOVEL REACTION IN METABOLISM

C-葡萄糖醛酸化——代谢中的新反应

• 批准号: 3280161
• 负责人: JOHN C CRAIG
• 金额: $ 8.8万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-12-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3280161
• 关键词: beta glucuronidase; biotransformation; glucuronides; glucuronosyltransferase; hydrolysis; steroid glycoside aglycone; uridine diphosphate

Recent work has indicated G-glucoronidation as a novel mammalian metabolic pathway. Since C-glucuronides are resistant to Beta-glucuronidase hydrolysis, they can not be identified by normal methods using this hydrolysis to regenerate the aglycone from the conjugate. This project is aimed at the examination of purified UDPG transferases to discover if the specific ability to conjugate at carbon is associated with different forms of UDPGT. At present the extent and degree of C-glucuronidation is not known as the enzymes carrying out this function have not been isolated. Since C-glucuronidation has been found to occur in phenolic structures and in some common drug molecules, this pathway is clearly of far greater general importance than has been realized heretofore, and has important implications in drug and xenobiotic detoxification mechanisms. Purified enzyme preparations will be examined for conjugating ability using the known substances found to give C-glucuronidation so far, and products will be isolated and characterized.

最近的研究表明G-葡萄糖苷酸化是一种新的哺乳动物代谢方式 路径。由于C-葡萄糖醛酸苷对β-葡萄糖醛酸苷酶具有抗药性 水解，它们不能用使用这种方法的正常方法识别 从结合物中重新生成苷元的水解法。这个项目是 目的是检测纯化的UDPG转移酶是否 碳结合的特殊能力与不同的形式有关 UDPGT.目前，C-葡萄糖醛酸化的程度和程度还不是 我们所知的执行这种功能的酶还没有被分离出来。 由于已发现C-葡萄糖醛酸化作用发生在酚类结构和 在一些常见的药物分子中，这一途径显然要大得多。 比到目前为止认识到的普遍重要性，并具有重要的 对药物和异种生物解毒机制的影响。纯净的 酶制剂的结合能力将使用 到目前为止，已发现的可导致C-葡萄糖醛酸化的物质和产品将 与世隔绝，独树一帜。