GENETIC BASIS OF SELECTION IN NATURAL POPULATIONS

自然种群选择的遗传基础

• 批准号: 3277831
• 负责人: Daniel E. DYKHUIZEN
• 金额: $ 16.92万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-09-01 至 1986-09-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3277831
• 关键词: Escherichia coli; aldehyde dehydrogenases; alleles; biochemical evolution; biological polymorphism; electrophoresis; exopeptidase; genetic manipulation; genetic strain; genotype; glucose 6 phosphate isomerase; isomerase; phosphoglucomutase; phosphogluconate dehydrogenase; transferase

Using appropriate genetic procedures for the production of isogenic strains of E. coli and appropriate media for positive and negative controls, we have successfully analyzed the selective effects of four alleles determining naturally occurring allozymes of the enzyme 6-phosphogluconate dehydrogenase. The alleles are selectively neutral in the K-12 genetic background. We now propose to carry out similar studies of loci associated with a number of other allozyme polymorphisms, including alcohol dehydrogenase, glutamic oxaloacetate transferase, glucose-6-phosphate dehydrogenase, phosphoglucose isomerase, glyceraldehyde-3-phosphate dehydrogenase, peptidase, mannose phosphate isomerase, isocitrate dehydrogenase, and phosphoglucomutase. Possible multiple locus effects on fitness will also be examined, as will certain biochemical characteristics of allozymes found to be associated with selection. These studies bear on the hypothesis of selective neutrality as it pertains to allozymes.

使用适当的遗传程序生产等基因菌株， E.大肠杆菌和适当的培养基作为阳性和阴性对照，我们有 成功地分析了四个等位基因的选择效应， 天然存在的6-磷酸葡萄糖酸脱氢酶的等位酶。 等位基因在K-12遗传背景中选择性中性。 我们现在 建议对与其他一些基因座相关的基因座进行类似的研究， 等位酶多态性，包括乙醇脱氢酶、谷氨酸草酰乙酸 转移酶，葡萄糖-6-磷酸脱氢酶，磷酸葡萄糖异构酶， 甘油醛-3-磷酸脱氢酶，肽酶，甘露糖磷酸 异构酶、异柠檬酸脱氢酶和磷酸葡萄糖变位酶。 可能的多 基因座对适应性的影响也将被研究，某些生化 发现与选择有关的等位酶的特征。 这些 研究表明，选择性中立的假设，因为它涉及 等位酶