RNA METABOLISM IN DEVELOPMENT AND HORMONAL REGULATION

发育和荷尔蒙调节中的 RNA 代谢

• 批准号: 3277987
• 负责人: LEON S DURE
• 金额: $ 16.22万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-08-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3277987
• 关键词: DNA directed RNA polymerase; Leguminoseae; RNA; autoradiography; cell cycle; cell growth regulation; endonuclease; eukaryote; gene expression; genetic manipulation; genetic mapping; genetic transcription; genetic translation; growth /development; hormone regulation /control mechanism; messenger RNA; molecular cloning; nucleic acid metabolism; nucleic acid sequence; plant growth /development; plant population genetics; polyadenylate; radiotracer; ribosomal RNA; tissue /cell culture

The proposed research focuses on elucidating mechanisms operative in the regulation of gene expression during normal development and hormone-induced growth transitions, emphasizing the transaction from quiescence to proliferation. The gene systems to be investigated are 1) selected abundant poly(A) mRNAs which are regulated (i.e. change up to 100-fold in concentration) during normal development and hormone-induced tissue proliferation and 2) rRNA the synthesis of which is rapidly and dramatically altered during these same growth transitions. The studies will encompass 1) experiments designed to elucidate the mechanism(s) by which the concentration of these hormone-responsive abundant poly(A) mRNAs is regulated and 2) and an analysis of the control of RNA polymerase I activity. Cloned cDNAs homologous to these abundant mRNAs will be used as hybridization probes to assess the contribution of altered rates of synthesis, processing and/or turnover to the dramatic change in concentration of the individual mRNAs, some of which increase and others which decrease in response to hormone treatment. The structure/organization of genomic clones of some of these auxin-responsive gene sequences will be investigated. Isolated nucleoli and a reconstituted system (including defined cloned rDNA templates, purified RNA polymerase I, and various crude and/or purified cellular extracts) will be used in studies directed to an analysis of the mechanisms operative in the extensive modulation of RNA polymerase I activity which occurs during various normal and hormone-induced growth transitions, emphasizing the transition to abnormal tissue proliferation. Factors which effect specific initiation and transcription of rRNA chains and which modulate the rate of transcription by engaged RNA polymerase I molecules will be sought and their status in quiescent and proliferating tissues investigated.

这项拟议的研究集中在阐明在脑血管疾病中的作用机制。 正常发育和激素诱导过程中基因表达的调控 增长转型，强调交易从静止到 扩散。要研究的基因系统是1)选择的 受调控的丰富的Poly(A)mRNAs(即，在 浓度)在正常发育和激素诱导的组织中 增殖和2)rRNA的合成是快速和 在这些相同的增长过渡期间发生了戏剧性的变化。这些研究 将包括1)旨在阐明机制的实验(S)，通过 这些激素反应丰富的聚(A)mRNAs的浓度 2)和RNA聚合酶I的调控分析 活动。与这些丰富的mRNAs同源的克隆的cDNA将被用作 杂交探针用于评估变异率的贡献 合成、加工和/或周转到戏剧性变化 单个mRNA的浓度，其中一些增加，另一些增加 对激素治疗的反应会降低。这个 其中一些对生长素反应的基因组克隆的结构/组织 将对基因序列进行调查。分离的核仁和重组的 系统(包括已定义的克隆rDNA模板、纯化的RNA聚合酶I、 和各种粗制和/或纯化的细胞提取物)将用于 研究旨在分析在脑血管疾病中起作用的机制 RNA聚合酶I活性的广泛调节，发生在 各种正常和激素诱导的生长过渡，强调 过渡到异常组织增殖。影响特定因素的因素 RRNA链的启动和转录，并调节 将寻求通过参与的RNA聚合酶I分子进行转录 研究它们在静止期和增殖期组织中的状态。