TIMING OF MAMMALIAN CELL CYCLE EVENTS

我们建议检查细胞计时的动力学， 同步和非同步动物细胞培养。 的假设 一个短周期的量子时钟负责细胞行为的特征 例如温度补偿和相位响应将被测试 使用数字视频显微镜，图像处理和流动 显微荧光测定法 时钟表现为极限环的概念 振荡器也将被探索。 细胞大小作为边界的作用 条件在振荡器的表达和可能性，这是 互动导致消极的母女关系和积极的姐妹关系 将测试细胞世代时间的相关性。 细胞夹带 除以生长因子的组合和温度变化， 尝试使用已知的细胞周期阶段对这些试剂的反应行为 作为背景。 细胞分裂的夹带的体内表达 生长因子水平的变化将被探索， 金色的。

We propose to examine the dynamics of cellular timekeeping using synchronous and non-synchronous animal cell cultures. The hypothesis that a short period, quantal clock is responsible for features of cell behavior such as temperature compensation and phase responsiveness will be tested using digital video microscopy, image processing and flow microfluorometry. The notion that the clock behaves as a limit cycle oscillator will also be explored. The role of cell size as a boundary condition in the expression of the oscillator and the possibility that this interaction leads to negative mother-daughter and positive sister-sister correlations in cell generation times will be tested. Entrainment of cell division by combinations of growth factors and by temperature shifts will be attempted using known cell cycle phase response behavior to these agents as a background. In vivo expression of entrainment of cell division by changing levels of growth factor will be explored using Mesocricetus auratus.