REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
批准号:
3278854
负责人:
SHARON R LONG
金额:
$21.58万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1982
资助国家:
美国
项目状态:
已结题
起止时间:
1982-07-01 至 1993-07-31
中文摘要
许多细菌建立致病或共生相互作用,
更高的生物。 我们研究的是
苜蓿根瘤菌与寄主豆科植物苜蓿相互作用
这种宿主-微生物联合通过一系列有序的
步骤,这些步骤是独特的,对相互作用具有高度特异性
特定的物种。 互动的早期步骤是
由一系列的细菌转化(nod)基因控制,
表达是由一种小的类黄酮化合物,毛地黄黄酮,
由主持人制作。 此外,nod基因的诱导依赖于
一种蛋白质,NodD。 In R.苜蓿属植物中,三个CCLD基因编码
具有可区分的大小和性质的调节蛋白。
本文综述了黄酮类诱导剂(毛地黄黄酮)、
调控上游DNA序列、NodD蛋白和RNA
将研究聚合酶。 将标记木犀草素,
受体蛋白鉴定。 NodD和NodD 3将被纯化,
确定了NodD介导黄酮的基础
诱导结瘤基因,而NodD 3引起组成型结瘤基因
表情 RNA聚合酶将被纯化,并专门
将分离和表征对nod基因的影响的因素
表情 删除分析将用于指示角色
诱导型nod基因上游的序列。 的存在
除了BHD之外的调节基因将被遗传测试,
这些基因的产物将被表征。 具体
假设NodD结合黄酮并激活转录
通过与DNA的直接相互作用,将被详细分析。
英文摘要
Many bacteria establish pathogenic or symbiotic interactions with
higher organisms. We study the nutritionally mutualistic
interaction or Rhizobium meliloti with a host legume, alfalfa.
This host-microbe association proceeds by a ordered series of
steps, which are unique and are highly specific to the interaction
of particular species. The early steps of the interaction are
controlled by a series of bacterial nodulation (nod) genes, whose
expression is induced by a small flavonoid compound, luteolin,
produced by the host. In addition, induction of nod genes depends
on a protein, NodD. In R. meliloti, three nodD genes encode
regulatory proteins with distinguishable sizes and properties.
The roles in induction of the flavone (luteolin) inducer, the
regulatory upstream DNA sequences, the NodD protein, and RNA
polymerase will be studied. Luteolin will be labelled and its
receptor protein identified. NodD and NodD3 will be purified and
characterized to determine the basis for NodD mediating flavone
induction of nod genes, while NodD3 causes constitutive nod gene
expression. RNA polymerase will be purified, and specialized sigma
factors will be isolated and characterized for effects on nod gene
expression. Deletion analysis will be used to indicate the roles
of sequences upstream of inducible nod genes. The existence of
regulatory genes besides nodD will be tested genetically, and the
products of such genes will be characterized. The specific
hypothesis that NodD binds flavone, and activates transcription
through direct interaction with DNA, will be analyzed in detail.
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Control of Symbiotic Gene Expression in Sinorhizobium meliloti
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批准号:8867256
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项目类别:
-
资助金额:$89.82万
-
财政年份:2010
-
负责人:SHARON R LONG
-
依托单位:
Control of Symbiotic Gene Expression in Sinorhizobium meliloti
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批准号:9069002
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项目类别:
-
资助金额:$84.12万
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财政年份:2010
-
负责人:SHARON R LONG
-
依托单位:
Control of Symbiotic Gene Expression in Sinorhizobium meliloti
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批准号:8249118
-
项目类别:
-
资助金额:$91.15万
-
财政年份:2010
-
负责人:SHARON R LONG
-
依托单位:
Control of Symbiotic Gene Expression in Sinorhizobium meliloti
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批准号:9274989
-
项目类别:
-
资助金额:$74.85万
-
财政年份:2010
-
负责人:SHARON R LONG
-
依托单位:
Control of Symbiotic Gene Expression in Sinorhizobium meliloti
-
批准号:7919006
-
项目类别:
-
资助金额:$92.2万
-
财政年份:2010
-
负责人:SHARON R LONG
-
依托单位:
Control of Symbiotic Gene Expression in Sinorhizobium meliloti
-
批准号:8733415
-
项目类别:
-
资助金额:$83.61万
-
财政年份:2010
-
负责人:SHARON R LONG
-
依托单位:
Control of Symbiotic Gene Expression in Sinorhizobium meliloti
-
批准号:8462636
-
项目类别:
-
资助金额:$88.45万
-
财政年份:2010
-
负责人:SHARON R LONG
-
依托单位:
Control of Symbiotic Gene Expression in Sinorhizobium meliloti
-
批准号:8073995
-
项目类别:
-
资助金额:$91.12万
-
财政年份:2010
-
负责人:SHARON R LONG
-
依托单位:
GORDON CONFERENCE ON MICROBIAL ADHESION AND SIGNALING
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批准号:2378442
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项目类别:
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资助金额:$0.2万
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财政年份:1997
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负责人:SHARON R LONG
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依托单位:
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
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批准号:6525688
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项目类别:
-
资助金额:$35.12万
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财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
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批准号:3484710
-
项目类别:
-
资助金额:$36.14万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
RHIZOBIUM NODULATION GENE EXPRESSION AND GENE PRODUCTS
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批准号:3278856
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项目类别:
-
资助金额:$16.51万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
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批准号:2175961
-
项目类别:
-
资助金额:$31.27万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
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批准号:2459343
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项目类别:
-
资助金额:$31.24万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
RHIZOBIUM NODULATION GENE EXPRESSION AND GENE PRODUCTS
-
批准号:3278857
-
项目类别:
-
资助金额:$25.56万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
-
批准号:6385452
-
项目类别:
-
资助金额:$33.93万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
-
批准号:3278859
-
项目类别:
-
资助金额:$21.28万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
-
批准号:2459342
-
项目类别:
-
资助金额:$35.15万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
-
批准号:2175963
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项目类别:
-
资助金额:$33.8万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
REGULATION OF NODULATION GENES IN RHIZOBIUM MELILOTI
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批准号:3278860
-
项目类别:
-
资助金额:$18.64万
-
财政年份:1982
-
负责人:SHARON R LONG
-
依托单位:
海外基金