REGULATION OF THE GAL GENES IN YEAST

这项研究的长期目标是了解分子 真核生物转录调控机制的研究进展 吉恩。为了实现这一目标，我们将分析转录的控制 利用半乳糖所需的酵母基因(半乳糖基因)，其中 两个关键的调控基因产物(GAL4和GAL80)控制着诱导性 在转录水平上的五个GAL结构基因。三 将进行相互关联的研究。第一个是聚焦 鉴定启动子功能和正确的DNA序列 三个GAL结构基因的调控。这涉及到分析 体外产生的突变对GAL-LacZ融合的影响。第二 一系列研究涉及构建高产克隆半乳糖的菌株 调控基因产物(GAL4和GAL80)，然后纯化和 这两种蛋白质的特性。第三条研究路线是 旨在开发一种转录酵母的转录系统 RNA聚合酶II在体外表达的基因。在工作的所有阶段，我们计划 将体内进行的实验结果与所进行的实验结果相关联 在试管中。希望这将使我们能够得出坚定的结论。 关于真核生物转录调控的分子机制。

The long-range goal of this research is to understand the molecular mechanisms involved in the transcriptional regulation of a eukaryotic gene. To achieve this goal, we will analyze the control of transcription of yeast genes required for galactose utilization (the GAL genes), in which two key regulatory gene products (GAL4 and GAL80) control the inducibility of five GAL structural genes at the level of transcription. Three interrelated lines of research will be conducted. The first is focused towards identifying DNA sequences required for promoter function and proper regulation of three GAL structural genes. This involves analyzing the affects of mutations generated in vitro on GAL-lacZ fusions. The second line of research involves constructing strains which overproduce cloned GAL regulatory gene products (GAL4 and GAL80), followed by purification and characterization of these two proteins. The third line of research is directed towards developing a transcription system for transcribing yeast genes by RNA polymerase II in vitro. At all stages of the work, we plan to correlate the results of experiments performed in vivo with those performed in vitro. This will, hopefully, enable us to draw firm conclusions concerning molecular mechanisms of transcriptional control in eukaryotes.