DNA REPLICATION IN YEAST

酵母中的 DNA 复制

• 批准号: 3284753
• 负责人: BIK-KWOON TYE
• 金额: $ 18.09万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-04-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3284753
• 关键词: DNA directed DNA polymerase; endonuclease; fungal genetics; gel electrophoresis; genetic manipulation; genetic mapping; genetic recombination; genetic transcription; molecular cloning; mutant; nucleic acid structure; radiotracer; spectrometry; tissue /cell culture; ultracentrifugation; uracil nucleoside

We are interested in using Saccharomyces cerevisiae as our model system to study chromosome replication in eukaryotes. By means of conventional genetics and current techniques developed in molecular cloning, we hope to dissect the complex chromosomes into simple replication units so as to: (1) study the structure, complexity and organization of individual replication initiation sites (replicators), and (2) examine the signals that activate replication at these sites. By gathering the information from these studies, we hope to learn something about the replication of chromosomes as a whole and the control mechanism which initiates their replication.

我们有兴趣使用酿酒酵母作为我们的模型系统来研究 真核生物中的染色体复制。 通过传统的遗传学方法， 目前的分子克隆技术发展，我们希望解剖 复杂的染色体转化为简单的复制单位，以便：（1）研究 单个复制启动结构、复杂性和组织 位点（复制因子），和（2）检查激活复制的信号， 这些网站。 通过收集这些研究的信息，我们希望了解 关于染色体的复制和控制 启动其复制的机制。