FIBEROPTIC-BASED FLUOROIMMUNO SENSORS

将开发独特的基于光纤的化学传感器来测量 药物、毒素和生命系统中自然产生的生物化学物质。 本提案描述和讨论了仪器和分析 将荧光免疫分析原理与激光技术相结合的方法学 光纤系统。所描述的荧光免疫传感器(FIS)源自它们的 利用抗体-抗原的特异性实现分析的选择性 反应。在这项工作中，抗体被固定在一个 FIS和化验程序中的光纤最终将涉及 体内孵育后进行体外荧光测量。高 灵敏度是通过综合利用Eu来实现的。 螯合荧光标记、脉冲激光激发和信号恢复 门控光子计数器。 旨在优化制备程序的初步体外研究 而使用FIS将使用一种商业上可用的免疫化学物质 系统。随后的实验涉及生产抗体以用于 某些化学致癌物，在FIS中加入这些抗体，以及 计划在实验动物身上使用这些FIS进行实际测量 以演示此工具的功能。一个 对拟议的实验将采取跨门徒计划的方法。 在这部作品中。

Unique fiberoptic-based chemical sensors will be developed to measure drugs, toxins, and naturally occurring biochemicals in living systems. This proposal describes and discusses instrumentation and analytical methodologies which integrate fluoroimmunoassay principles with laser-based fiberoptic systems. The described fluoroimmuno sensors (FIS) derive their analytical selectivity through the specificity of antibody-antigen reactions. In this work antibodies are immobilized at the terminus of a fiberoptic within the FIS and assay procedures will ultimately involve in-vivo incubation followed by in-vitro fluorescence measurement. High sensitivity is to be achieved through the combined utilization of europium chelate fluorotags, pulsed laser excitation, and signal recovery using a gated photon counter. Initial in-vitro studies aimed at optimizing the procedures for preparing and using the FIS will employ a commercially available immunochemical system. Subsequent experiments involving the production of antibodies for certain chemical carcinogens, incorporation of these antibodies in FIS, and use of these FIS for actual measurements in laboratory animals are planned to demonstrate the capabilities of this instrumentation. An interdisciplanary approach to the proposed experiments will be pursued throughout this work.