STUDIES ON MECHANISTICALLY CRYPTIC ENZYMATIC REACTIONS

脂环中两种微生物酶的作用机理 新陈代谢将从立体化学的角度进行研究。环己酮 加氧酶是一种黄素蛋白，它催化微生物版本的 Baeyer-Villager反应，在助剂下将环酮转化为内酯 分子氧的能量。该酶已被分离和鉴定；然而 它的作用机制仍然是一个有很多猜测的问题，就像 大多数需要黄素的酶。环丙烷羧酸水解酶催化 环丙烷羧酸肉碱衍生物的水解环断裂 酸，最终得到4-羟基丁酸。这种酶还没有被 纯化，对其作用机制知之甚少。 建议通过对这两种酶的研究来研究它们的作用机制。 底物和产物之间的立体化学关系。涉案遗嘱 Be：(A)区域和立体特异性标记底物的合成 氚，以及(B)手性标记的化学降解和酶分析 产品。黄曲霉毒素研究的药学和环境意义 讨论了这种分解代谢过程。

The mechanisms of action of two microbial enzymes involved in alicyclic ring metabolism will be studied from a stereochemical point of view. Cyclohexanone oxygenase is a flavoprotein which catalyzes, a microbiological version of the Baeyer-Villager reaction, transforming cyclic ketones into lactones with the aid of molecular oxygen. The enzyme has been isolated and characterized; however its mechanism of action is still a matter of much speculation, as is true for most flavin-requiring enzymes. Cyclopropane carboxylate hydrolase catalyzes the hydrolytic ring cleavage of the carnitine derivative of cyclopropane carboxylic acid, ultimately affording 4-hydroxybutyric acid. The enzyme has not been purified, and very little is known regarding its mechanism of action. It is proposed to study the mechanisms of both enzymes through investigation of the stereochemical relationships between substrates and products. Involved will be: (a) synthesis of substrates regio- and stereospecifically labeled with tritium, and (b) chemical degradation and enzymic analysis of chirally-labeled products. The pharmaceutical and environmental significance of the study of such catabolic processes is discussed.