SECRETORY PROTEINS IN THE MALE REPRODUCTIVE SYSTEM

我们小组以前的工作导致了对这种病毒的发现、分离和 几种不同节段特异性分泌蛋白的表征 男性生殖系统，并认为是重要的实现 精子的完全成熟和活力。 其中三种蛋白质， 雄激素结合蛋白（ABP）、酸性附睾糖蛋白（AEG）和 前列腺类固醇结合蛋白，前列腺素，将详细研究依赖 在我们实验室开发的针对高度纯化的 从大鼠组织中分离的抗原。 蛋白质会定位在组织中 光镜和电镜免疫细胞化学（ICC）切片。 在 此外，放射免疫分析（RIA）将用于定量评估 三种蛋白质的组织含量和血液水平。 ICC和RIA 技术将用于研究细胞和亚细胞分布， 分泌机制，个体发育，激素调节，男性命运 生殖道，以及可能的人工控制这些分泌 雄性生殖系统分泌的器官特异性雄激素依赖性蛋白 道。 这些研究将首次提供具体的见解， 男性生殖系统中的蛋白质分泌机制，并可能提供 人工控制男性生育能力的新可能性。

Previous work in our group resulted in the discovery, isolation, and characterization of several secretory proteins specific for different segments of the male reproductive system, and believed to be important in the attainment of full maturation and motility of sperm. Three of these proteins, testicular androgen binding protein (ABP), acidic epididymal glycoprotein (AEG), and a prostatic steroid binding protein, prostatein, will be studied in detail relying on specific antisera developed in our laboratories against highly purified antigens isolated from rat tissues. The proteins will be localized in tissue sections with both light and electron microscopic immunocytochemistry (ICC). In addition, radioimmunoassays (RIA) will be developed for quantitative estimation of the tissue contents and blood levels of all three proteins. Both ICC and RIA techniques will be used to study the cellular and subcellular distribution, the secretory mechanisms, ontogeny, hormonal regulation, fate in the male reproductive tract, and possible artificial control of secretion of these organ-specific, androgen dependent proteins secreted in the male reproductive tract. These studies will provide, for the first time, specific insights into the protein secretory mechanisms in the male reproductive system, and may offer novel possibilities for the artificial control of male fertility.