SECRETORY PROTEINS IN THE MALE REPRODUCTIVE SYSTEM

我们小组之前的工作导致了发现、隔离和 几种不同片段特异性分泌蛋白的特性分析 男性生殖系统的，并被认为在达到 精子的完全成熟和能动性。其中三种蛋白质是睾丸 雄激素结合蛋白(ABP)、酸性附睾糖蛋白(AEG)和 前列腺类固醇结合蛋白，前列腺素，将依赖于 我们实验室研制的抗高纯度特异性抗血清的研究 从大鼠组织中分离出的抗原。这些蛋白质将定位于组织中 光镜和电子显微镜免疫细胞化学(ICC)切片。在……里面 此外，还将开发放射免疫分析(RIA)用于定量评估 所有三种蛋白质的组织含量和血液水平。ICC和RIA 技术将被用来研究细胞和亚细胞的分布， 雄性的分泌机制、个体发育、激素调节、命运 生殖道，以及可能的人工控制这些分泌物 雄性生殖器分泌的器官特异性雄激素依赖蛋白 一条小路。这些研究将第一次提供对 男性生殖系统中的蛋白质分泌机制，并可能提供 人工控制男性生育能力的新可能性。

Previous work in our group resulted in the discovery, isolation, and characterization of several secretory proteins specific for different segments of the male reproductive system, and believed to be important in the attainment of full maturation and motility of sperm. Three of these proteins, testicular androgen binding protein (ABP), acidic epididymal glycoprotein (AEG), and a prostatic steroid binding protein, prostatein, will be studied in detail relying on specific antisera developed in our laboratories against highly purified antigens isolated from rat tissues. The proteins will be localized in tissue sections with both light and electron microscopic immunocytochemistry (ICC). In addition, radioimmunoassays (RIA) will be developed for quantitative estimation of the tissue contents and blood levels of all three proteins. Both ICC and RIA techniques will be used to study the cellular and subcellular distribution, the secretory mechanisms, ontogeny, hormonal regulation, fate in the male reproductive tract, and possible artificial control of secretion of these organ-specific, androgen dependent proteins secreted in the male reproductive tract. These studies will provide, for the first time, specific insights into the protein secretory mechanisms in the male reproductive system, and may offer novel possibilities for the artificial control of male fertility.