REGULATION OF ANDROGEN SECRETION IN MAMMALIAN TESTES

哺乳动物睾丸雄激素分泌的调节

• 批准号: 3310652
• 负责人: LARRY L. EWING
• 金额: $ 19.07万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-02-01 至 1986-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310652
• 关键词: Leydig cells; androgen inhibitor; busulfan; cytology; cytoplasm; drug administration routes; electron microscopy; epididymis; epithelium; estradiol; hormone regulation /control mechanism; isolation perfusion; luteinizing hormone; organelles; pregnenolone; radioimmunoassay; reproductive system pharmacology; spermatogenesis inhibitor; steroid hormone biosynthesis; testosterone

During the past year we completed a series of experiments comparing testosterone secretion by rat, rabbit, dog, guinea pig and hamster testes perfused in vitro. Species differences in testosterone secretion were discovered. These species differences in testosterone secretion were found not to be correlated with the mass of Leydig cells present in the testis. Instead, testosterone secretion was correlated directly with the amount of smooth endoplasmic reticulum in Leydig cells. Testosterone secretion and the proportion of Leydig cell smooth endoplasmic reticulum was highest in the guinea pig followed in order by rabbit, dog, rat and hamster. Finally, it was shown that the flux of steroids through the testosterone biosynthetic pathway was markedly different in rats and rabbits. Apparently, rabbit testes perfused in vitro synthesized testosterone from pregnenolone via 17 hydroxypregnenolone yields dehydroepiandrosterone yields delta 5 androstenediol yields testosterone. By contrast, two pathways were found to predominate in rat testes perfused in vitro. Pregnenolone yields 17 hydroxypregnenolone yields 17 hydroxyprogesterone yields delta 4 androstenedione yields testosterone and pregnenolone yields progesterone yields 17 hydroxyprogesterone yields delta 4 androstenedione yields testosterone.

在过去的一年里，我们完成了一系列的实验， 体外灌注的大鼠、兔、犬、豚鼠和仓鼠睾丸分泌物。 发现睾酮分泌存在物种差异。 这些物种 睾丸激素分泌的差异被发现与 睾丸中存在大量间质细胞。 相反，睾丸激素分泌 与睾丸间质细胞中的滑面内质网数量直接相关 细胞 睾丸间质细胞的分泌与间质细胞的比例 内质网在豚鼠中最高，其次是兔， 狗、老鼠和仓鼠。 最后，研究表明，类固醇通过 大鼠的睾酮生物合成途径明显不同， 家兔 显然，体外灌注的兔子睾丸合成了睾酮 由双烯醇酮经17羟基双烯醇酮产生脱氢表雄酮 产生δ 5雄烯二醇产生睾酮。 相比之下， 发现在体外灌注的大鼠睾丸中占主导地位。 孕烯醇酮产量 17羟孕酮产生δ 4 雄烯二酮产生睾丸激素，双烯醇酮产生孕酮 17羟孕酮产生δ 4雄烯二酮产生睾酮。