RNA IN DNA VIRUS ASSEMBLY

一个120个核苷酸的噬菌体phi29 rna具有结构和 病毒前体壳(Prohead)的催化成分 其中包装的18kb碱基对双链病毒DNA 在一种高效的、依赖于ATP的体外反应中。RNA是一种 前部(连接件)的门式顶点的一部分， 与phi29 DNA包装蛋白gp16一起可以 作为DNA-GP3转位所需的ATPase发挥作用 (phi29 DNA在每个5‘端都有一个共价结合的蛋白GP3 总站)。因此，phi29 DNA“包装机”包括 连接蛋白(Gp10)、RNA、gp16，可能还有 DNA-GP3。RNA在序列起始和转录中的作用 DNA-GP3体外包装的易位事件及其意义 可能的功能作为ATPase的一部分将被调查。 将尝试将连接器中的RNA本地化 电子显微图像的处理与三维 重建。Phi29亲缘关系和其他基因的前导RNA 将对噬菌体进行分离和测序。高阶结构 Phi29前体RNA和对应RNA的研究将通过 使用比较序列分析、足迹和 化学和酶探针。

A 120 nucleotide bacteriophage PHI29 RNA is structural and catalytic constituent of the viral precursor shell (prohead) into which the 18 kilobase pair double-stranded viral DNA is packaged in an efficient, ATP-dependent, in vitro reaction. The RNA is a part of the portal vertex of the prohead (connector), which together with the PHI29 DNA packaging protein gp16 may function as an ATPase needed for translocation of DNA-gp3 (PHI29 DNA has a covalently bound protein gp3 at each 5' terminus). Thus the PHI29 DNA "packaging machine" includes the connector protein (gp10), RNA, gp16, and perhaps the gp3 of DNA-gp3. The role of the RNA in the sequential initiation and translocation events of in vitro DNA-gp3 packaging and its possible function as part of the ATPase will be investigated. Localization of the RNA in the connector will be attempted by processing of electron micrographs and three-dimensional reconstructions. Prohead RNAs of PHI29 relatives and other phages will be isolated and sequenced. The higher-order structure of PHI29 prohead RNA and counterpart RNAs will be studied by the use of comparative sequence analysis, footprinting, and chemical and enzymatic probes.