INTEGRATED SOLID-STATE/SOLUTION NMR STUDY OF DNA

DNA 综合固态/溶液核磁共振研究

• 批准号: 3295901
• 负责人: GERARD STANISLAUS HARBISON
• 金额: $ 12.95万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1996-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3295901
• 关键词: DNA; RNA; artificial intelligence; bioengineering /biomedical engineering; computer program /software; computer system design /evaluation; conformation; dipole moment; mathematical model; model design /development; nonwater solvent; nuclear magnetic resonance spectroscopy; nucleic acid chemical synthesis; nucleic acid structure; polymerase chain reaction; solid state; solutions

It is proposed to use a combination of solid-state and solution NMR spectroscopy to investigate the structure and dynamics of DNA and of drugs and other agents bound to DNA. It has recently been shown that the methods for determining the conformation of the deoxyribose ring in DNA oligomers may be susceptible to errors arising from quantum-mechanical interference between dipolar cross-relaxation and the J coupling. This J coupling is used, via the Karplus equation, to determine the conformation. A new mathematical formalism is proposed to allow calculation of these interference effects. A series of experiments is proposed to evaluate the seriousness of the errors resulting from a neglect of this term; methods for correct extraction of J couplings and cross-relaxation rate constants will be developed. Solid-state NMR experiments on DNA fibers and model compounds, which originally gave the discrepant results which pointed out the existence of this interference term, will be continued, with an emphasis on observing drug binding to synthetic DNA high polymers of high molecular weight.

建议采用固态核磁共振和溶液核磁共振相结合的方法