CARBOHYDRATE BINDING PROTEIN 35
CARBOHYDRATE BINDING PROTEIN 35
批准号:
3295374
负责人:
JOHN L WANG
金额:
$9.38万
依托单位国家:
美国
项目类别:
财政年份:
1988
资助国家:
美国
项目状态:
已结题
起止时间:
1988-04-01 至 1993-03-31
关键词:
N acetylglucosamine affinity chromatography binding proteins cell nucleus complementary DNA cytoplasm galactose genetic manipulation genetic transcription heterogeneous nuclear ribonucleoprotein laboratory mouse laboratory rabbit laboratory rat messenger RNA molecular cloning phosphoproteins protein sequence radiotracer ribonucleoproteins
中文摘要
本项目拟研究的是
碳水化合物结合蛋白35(Mr 35,000),一种结合凝集素的
特别是半乳糖-N-乙酰氨基葡萄糖(Gal-GlcNAc)
含有糖共轭化合物的。使用一种高度特异的抗体
针对CBP35,我们进行了引人注目的观察
CBP35可以在细胞的细胞核中找到,而且
CBP35的表达水平及其核定位可能是
取决于细胞的增殖状态。最近,
我们已经积累的证据表明CBP35是一种蛋白质
与非均相核的核心粒子有关
核糖核蛋白复合体(HnRNP)。
拟议研究的具体目标是:
(A)鉴定、分离和鉴定cDNA和基因组
CBP35克隆;
(B)测定CBP35的部分氨基酸序列
蛋白质水平并推导出完整的氨基酸序列
从cDNAs序列中克隆出;
(C)研究CBP35基因转录调控。
北方分析和核补给实验;
(D)分析CBP35是否有可能不同
核型与细胞质的关系,以及它们与
CBP35的不同等电变异体及其不同状态
磷酸化;
(E)搜索将CBP35从
HnRNP核心粒子与识别之间的对应关系
CBP35和核心颗粒的蛋白质(S);
(F)分析CBP35在一定扰动下的影响
试验(例如,添加CBP35和/或兔抗CBP35)
无细胞信使核糖核酸加工(剪接)系统。
所有这些研究都应该有助于我们理解
动物细胞内源性凝集素的结构和活性。
英文摘要
This project propose to study the structure and activities of
Carbohydrate-Binding Protein 35 (Mr 35,000), a lectin that binds
specifically to galactose-N-acetylglucosamine (Gal-GlcNAc)
containing glycoconjugates. Using a highly specific antibody
directed against CBP35, we have made the striking observation
that CBP35 can be found in the nucleus of a cell and that the
level of expression of CBP35 and its nuclear localization may be
dependent on the proliferation state of the cell. More recently,
we have accumulated evidence to indicate that CBP35 is a protein
associated with the core particle of the heterogeneous nuclear
ribonucleoprotein complex (hnRNP).
The specific objectives of the proposed research are:
(a) to identify, isolate, and characterize cDNA and genomic
clones for CBP35;
(b) to determine the partial amino acid sequence of CBP35 at the
protein level and to derive the complete amino acid sequence
from the cDNA sequence;
(c) to study the regulation of transcription of the CBP35 gene by
Northern analysis and nuclear run-on experiments;
(d) to analyze CBP35 in terms of the possibility of different
nuclear versus cytoplasmic forms, their relationships to the
different isoelectric variants of CBP35 and the different states of
phosphorylation;
(e) to search for conditions that will release CBP35 from the
hnRNP core particle and to identify the correspondence between
CBP35 and the protein(s) of the core particle; and
(f) to analyze the effect of CBP35 in certain perturbation
experiments (e.g. addition of CBP35 and/or rabbit anti-CBP35) on
cell free mRNA processing (splicing) systems.
All of these studies should contribute to our understanding of the
structure and activities of lectins endogenous to animal cells.
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PHOSPHORYLATION OF CARBOHYDRATE BINDING PROTEIN 35
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批准号:6248443
-
项目类别:
-
资助金额:$0.46万
-
财政年份:1997
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE BINDING PROTEIN 35
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批准号:6258871
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项目类别:
-
资助金额:$0.05万
-
财政年份:1997
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE-BINDING PROTEIN 35
-
批准号:2179503
-
项目类别:
-
资助金额:$20.11万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE-BINDING PROTEIN 35
-
批准号:6179536
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项目类别:
-
资助金额:$22.22万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE BINDING PROTEIN 35
-
批准号:3295375
-
项目类别:
-
资助金额:$9.58万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE BINDING PROTEIN 35
-
批准号:3295376
-
项目类别:
-
资助金额:$9.55万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE BINDING PROTEIN 35
-
批准号:3295372
-
项目类别:
-
资助金额:$9.01万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
Carbohydrate Binding Protein 35
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批准号:6694083
-
项目类别:
-
资助金额:$26.31万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE-BINDING PROTEIN 35
-
批准号:2684862
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项目类别:
-
资助金额:$20.96万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE BINDING PROTEIN 35
-
批准号:3295377
-
项目类别:
-
资助金额:$9.93万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE-BINDING PROTEIN 35
-
批准号:2179500
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项目类别:
-
资助金额:$12.38万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE-BINDING PROTEIN 35
-
批准号:3295373
-
项目类别:
-
资助金额:$11.83万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
Carbohydrate Binding Protein 35
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批准号:6573243
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项目类别:
-
资助金额:$26.01万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE-BINDING PROTEIN 35
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批准号:2022174
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项目类别:
-
资助金额:$20.86万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE-BINDING PROTEIN 35
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批准号:2179502
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项目类别:
-
资助金额:$19.36万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE-BINDING PROTEIN 35
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批准号:2900658
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项目类别:
-
资助金额:$21.58万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
Carbohydrate Binding Protein 35
-
批准号:6838253
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项目类别:
-
资助金额:$26.31万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
Carbohydrate Binding Protein 35
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批准号:7002199
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项目类别:
-
资助金额:$25.69万
-
财政年份:1988
-
负责人:JOHN L WANG
-
依托单位:
CARBOHYDRATE BINDING PROTEIN 35
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批准号:2179501
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项目类别:
-
资助金额:$4.6万
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财政年份:1988
-
负责人:JOHN L WANG
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依托单位:
ACTION OF LYSOSOMAL ENZYMES ON THE EXTRACELLULAR MATRIX
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批准号:3281031
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项目类别:
-
资助金额:$6.62万
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财政年份:1983
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负责人:JOHN L WANG
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依托单位:
海外基金