STRUCTURE AND EXPRESSION OF EUCARYOTIC GENES

人们建议用实验来阐明基因之间的关系。 线虫的组织、结构和表达 优雅女装。线虫之所以被使用，是因为它具有可用的遗传学，体型小 基因组和描述良好的细胞谱系。白纹伊蚊的基因组排列 肌动蛋白和胶原基因正在确定，DNA序列分析是 正在进行中。肌动蛋白和胶原基因的表达时间将 用克隆的序列作为杂交探针进行检测。 还将用原位杂交方法检测BAE在特定、 很好地描述了细胞谱系。将进行实验以确定是否 线虫肌动蛋白基因可以在酵母中表达并发挥作用 评估肌动蛋白基因的保守性。线虫肌动蛋白和线虫 胶原蛋白克隆的DNA正在通过两个杂交进行遗传作图 含有限制性内切酶切点多态性的菌株。更多的多态 将在其他实验室和野生株中寻找以改进作图 方法。一些DNA多态是由于DNA插入造成的，它们将是 研究以确定它们是否为可转座的遗传元件。当 肌动蛋白和胶原基因已被定位，突变体将用于研究 细菌中基因与蛋白质功能的关系 有机体。

Experiments are proposed to elucidate the relationships between gene organization, structure and expression in the nematode, Caenorhabditis elegans. The nematode is used because of its available genetics, small genome and well described cell lineages. The genomic arrangement of the actin and collagen genes is being determined and DNA sequence analysis is in progress. The times of expression of the actin and collagen genes will be determined using the cloned sequences as hybridization probes. Expression will also bae measured by in situ hybridization in specific, well-characterized cell lineages. Experiments will be done to determine if C. elegans actin genes can be expressed and function in yeast in order to assess the conservation of the actin genes. The C. elegans actin and collagen cloned DNA's are being genetically mapped using two interbreeding strains that contain restriction site polymorphisms. More polymorphisms will be sought in other laboratory and wild strans to improve the mapping methds. Some DNA polymorphisms are due to DNA inserts and they will be studied to determine if they are transposable genetic elements. When the actin and collagen genes are mapped, mutants will be used to study the relationship between the genes and the functions of the proteins in the organism.