STRUCTURE & FUNCTION OF THE EPIDIDYMIS AND VAS DEFERENS

结构

• 批准号: 3311733
• 负责人: DAVID W HAMILTON
• 金额: $ 10.94万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-29 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3311733
• 关键词: O glycosidase; Sertoli cells; antimetabolites; blood proteins; chorionic gonadotropin; corticosterone; epididymis; epithelium; estradiol; follicle stimulating hormone; galactosyltransferases; gel electrophoresis; genital secretion; hormone regulation /control mechanism; immunoelectrophoresis; isolation perfusion; membrane activity; organelles; pituitary gonadal axis; prolactin; radiotracer; secretion; sperm; sperm motility; testosterone

The primary goals of this application are to elucidate mechanisms of maturation of spermatozoa in the epididymis which will lead to an understanding of how fertility is regulated in the male. Maturation of sperm in the epididymis involves interactions among sperm, luminal fluid, and the epididymal epithelium (which is regulated by factors from the blood vascular system). In the experiments to be done in this application, investigation will be undertaken on proteins which are synthesized and secreted by the epididymal epithelium. Antibodies raised against these proteins cross-react with molecules on the sperm surface as well as with molecules that are derived from the testis. The first aim of this grant proposal is to isolate pure compounds from rete testis fluid, from the epididymis, and from sperm surfaces, which will then be studied extensively with respect to their amino acid sequences and the carbohydrate components of their oligosaccharide chains. The underlying theme of these experiments is to determine whether all of the molecules that cross-react immunologically are actually structurally the same, and whether they are the same as the Alpha-lactalbumin from rat milk. After deducing their structure, the molecules will be studied with respect to their ability to modify galactosyltransferase activity, which is a major function of Alpha-lactalbumin. The third aim in the project is related to studies on the synthesis and secretion (and control of these two functions) of immunologically cross-reactive molecules from the epididymis in culture. We have developed a perifusion culture system for epididymal tubules that will allow us to do studies on long-term cultures of epididymis, in which we know that epididymal epithelium and sperm are reasonably normal. Finally, using the well characterized monoclonal antibodies raised against the individual polypeptides of the epididymal Alpha-lactalbumin complex, experiments will be done to determine whether the molecules have any effect on sperm motility or fertilizing ability.

本申请的主要目的是阐明 精子在附睾中成熟，这将导致 了解男性的生育力是如何调节的。 成熟 附睾中的精子涉及精子，腔液， 以及附睾上皮（其由来自血液的因子调节 血管系统）。 在本申请中要进行的实验中， 将对合成的蛋白质进行研究， 由附睾上皮分泌。 针对这些病毒的抗体 蛋白质与精子表面的分子以及 来自睾丸的分子。 这笔赠款的第一个目的是 建议是从睾丸网液中分离纯化合物， 附睾，精子表面，这将被广泛研究 相对于它们的氨基酸序列和碳水化合物组分， 它们的寡糖链。 这些实验的潜在主题 是确定是否所有的交叉反应分子 在免疫学上实际上在结构上是相同的， 与鼠奶中的α-乳白蛋白相同。 在推断出他们的 结构，分子将研究其能力， 修饰半乳糖基转移酶活性，这是 α-乳白蛋白 该项目的第三个目标是研究 合成和分泌（以及这两种功能的控制） 来自培养的附睾的免疫交叉反应分子。 我们已经建立了一个附睾管灌流培养系统， 将使我们能够对附睾进行长期培养的研究， 我们知道附睾上皮和精子是相当正常的。 最后，使用充分表征的单克隆抗体， 附睾α-乳白蛋白复合物的单个多肽， 将进行实验以确定这些分子是否有任何影响， 对精子活力和可移植性的影响