HORMONAL REGULATION OF LUTEAL CELL DIFFERENTIATION

黄体细胞分化的激素调节

• 批准号: 3310636
• 负责人: PAUL LANDIS KEYES
• 金额: $ 10.99万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-03-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310636
• 关键词: autologous transplantation; cell differentiation; corpus luteum; cyclic AMP; estradiol; graafian follicles; hormone regulation /control mechanism; hypophysectomy; luteinizing hormone; progesterone; prolactin; radioimmunoassay; radiotracer; steroid hormone biosynthesis; testosterone; tissue /cell culture

The overall objective of this research is to investigate the endocrine regulation of cell differentiation and of progesterone synthesis in the corpus luteum of the rat and rabbit. Specifically, the physiological role of the theca lutein cell and the role of estrogen and of LH in the differentiation of the granulosa lutein cell will be emphasized. Two basic approaches, recently developed in this laboratory, will be used. First, transplants of luteinizing granulosa cells or of luteinizing whole follicles will be made into hypophysectomized Fischer 344 rats and in rabbits. Second, granulosa lutein cells from rabbits will be studied in monolayer cultures. The following hypotheses will be tested: 1) androgen (or its aromatized derivative) and prolactin are necessary for increased steroidogenic capacity and further differentiation of rat granulosa lutein cells in the second week after ovulation; 2) an important role of the theca lutein cell is its participation in luteal estrogen synthesis, which is stimulated by LH (rat); 3) the small steroidogenic cell population in rat and rabbit corpora lutea is derived from thecal cells of the follicle; 4) the role of LH in the rabbit corpus luteum is to stimulate the recruitment or differentiation of small luteal cells, suspected to be of thecal origin; 5) differentiation and progesterone synthesis by granulosa lutein cells in culture can be stimulated either by LH or by estradiol; the steroidogenic effect of estradiol is mediated by 3'5' - cyclic AMP (rabbit). These studies are expected to lead to new understanding of the interactions between cell populations in the corpus luteum and of the hormones that govern the normal function and regression of the corpus luteum.

本研究的总体目标是研究内分泌 细胞分化和孕酮合成的调节 大鼠和家兔的黄体。 具体来说，生理作用 以及雌激素和LH在卵泡膜黄体细胞中的作用。 将强调颗粒黄体细胞的分化。 两个基本 将使用本实验室最近开发的方法。 第一、 促黄体化颗粒细胞或促黄体化整体细胞的移植 将卵泡制成垂体切除的Fischer 344大鼠， 家兔 第二，来自兔子的颗粒黄体细胞将被研究， 单层培养 将检验以下假设：1）雄激素 (or其芳香化衍生物）和催乳素是增加 大鼠颗粒黄体生成激素能力及其进一步分化 排卵后第二周的卵泡膜细胞; 2）卵泡膜的重要作用 黄体细胞参与黄体雌激素的合成， LH刺激（大鼠）; 3）大鼠中的小类固醇生成细胞群 兔黄体来源于卵泡的卵泡膜细胞; 4） LH在兔黄体中的作用是刺激募集 或小黄体细胞分化，怀疑是卵泡膜起源; 5)卵巢颗粒黄体细胞的分化和孕酮的合成 培养物可由LH或雌二醇刺激;类固醇生成 雌二醇的作用由3 '5' -环AMP（兔）介导。 这些 这些研究有望使人们对这种相互作用有新的认识。 在黄体细胞群和激素之间， 控制黄体的正常功能和退化。