CHARACTERIZATION OF ARABIDOPSIS TELOMERES

拟南芥端粒的表征

• 批准号: 3302569
• 负责人: ERIC JEAN RICHARDS
• 金额: $ 12.85万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3302569
• 关键词: DNA; DNA replication; angiosperms; chemical structure function; chromosome disorders; chromosomes; eukaryote; gel electrophoresis; genetic mapping; genome; molecular cloning; mutant; nucleic acid probes; nucleic acid repetitive sequence; plant genetics; telomere; trisomy

This proposal is a part of a long-term project to understand the organization of higher eukaryotic genomes and the molecular biology of functional chromosome components, such as telomeres and centromeres. Telomeres are the specialized structures that form the termini of eukaryotic chromosomes. These structures are essential for the replication and maintenance of the genome. Telomeres have been extensively studied in lower eukaryotes but considerably less in known about higher eukaryotic telomeres. We have recently isolated a higher eukaryotic telomere from the flowering plant Arabidopsis thaliana, providing the opportunity to study the telomeric regions of this organism as a model system. Two aspects of telomere structure and function will be studied. First, the nature and organization of the DNA sequences that lie adjacent to A. thaliana telomeres will be investigated. Second, the mechanism by which a broken chromosome end acquires a new telomere (i.e., telomere healing) will be studied using a chromosomal mutant of A. thaliana which carries a healed telomere. The healed telomere will be isolated and its structure compared to that of the original chromosomal site to determine how the healing event took place. In addition, the healed telomere, which resides proximal to a centromere, will be investigated as a potential tool for studying A. thaliana centromere structure.

这个建议是一个长期项目的一部分，以了解