MODE OF ACTION OF 6-MBOA AS A REPRODUCTIVE STIMULATOR

6-甲氧基苯并恶唑啉酮（6-MBOA）是一种化合物，最初从 自然生长的植物刺激所有哺乳动物的繁殖， 远测试。 6-MBOA导致两种性别的性腺肥大， microtine啮齿动物Microtus montanus（脊椎动物）， 加强生殖工作。 6-MBOA管理克服了 光周期对M. montanus，似乎 在提示生殖行为方面比光周期更重要。 6-MBOA现在已经被公认为一种生殖兴奋剂和生育力 增强剂;然而，没有关于其作用机制的信息， 内分泌或生化水平。 我们的初步研究表明，6-MBOA 是褪黑激素拮抗剂，因为它竞争性地取代3 H-褪黑激素 其特异性地与蛋白质受体结合。 褪黑激素影响 人类和其他动物的性发育，可能是一种生物学上的 影响心理抑郁的因素 本提案中所述的研究旨在作为 全面研究确定6-MBOA对神经内分泌的影响 系统及其在细胞和生物化学水平上的作用机制。 本项目的目标是：（1）确定主要的 和6-MBOA的次级靶组织，通过测量组织分布 和体外结合，（2）测量促性腺激素LH和FSH的变化 通过放射免疫测定法测定6-MBOA给药后，（3）测定 6-MBOA对某些血清类固醇水平的影响;（4）研究可能的6-MBOA 和褪黑激素介导的生物化学变化。 所使用的方法是既定的和客观的。 结果 确定6-MBOA对神经内分泌系统的影响。

6-Methoxybenzoxazolinone (6-MBOA) is a compound originally isolated from naturally growing plants which stimulates reproduction in all mammals so far tested. 6-MBOA causes gonadol hypertrophy in both sexes of the microtine rodent Microtus montanus (vertebrates) with a commensurate increase in reproductive efforts. 6-MBOA administration overcomes the suppressive effects that photoperiods have on M. montanus, and appears to be more important than photoperiods in cueing reproductive behavior. 6-MBOA is now well established as a reproductive stimulant and fertility enhancer; however, there is no information on its mechanism of action at the endocrine or biochemical level. Our initial studies show that 6-MBOA is a melatonin antagonist in that it competitively displaces 3H-melatonin which is specifically bound to protein receptors. Melatonin influences sexual development in humans and other animals and is likely a biological factor influencing psychological depression. Research described in this proposal is intended as the initial phase of a comprehensive study to define effects of 6-MBOA on the neuroendocrine system and its mechanism(s) of action at the cellular and biochemical level. The objectives of the project described here are to: (1) determine primary and secondary target tissues for 6-MBOA by measuring tissue distributions and in vitro binding, (2) measure changes in the gonadotropins LH and FSH by radioimmunoassay after 6-MBOA administration, (3) determine effects of 6-MBOA on the levels of some serum steroids, and (4) study possible 6-MBOA and melatonin-mediated biochemical changes in established target tissues. Methodologies to be used are well established and objective. Results are certain to determine effects of 6-MBOA on the neuroendocrine system.