NEUROCHEMISTRY OF GESTATION-PRODUCED ANALGESIA

拟议研究的总体目标是确定 负责升高的外周和中枢过程 对观察到的厌恶刺激的反应阈值 大鼠妊娠期间。 将使用的一种方法是 确定模仿和操纵特定方面的效果 怀孕状况对大鼠疼痛阈值的影响。 第二种方法将 确定母血和脑脊液（CSF） 妊娠期间阿片肽的浓度分布。 具体的 目标是 (1) 确定雌激素、孕激素的作用（如果有） 和催乳素，单独或组合，对疼痛阈值 未怀孕的卵巢切除大鼠。 (2) 判断是否存在高程 在假怀孕期间观察疼痛阈值，无论是否有 蜕膜瘤。 (3)确定宫内压是否升高 膨胀足以使疼痛阈值增加 假孕大鼠和去势大鼠接受激素治疗 治疗。 (4) 确定是否存在发育中的胎儿 对于提高母亲疼痛阈值至关重要。 (5) 确定 B-内啡肽和总阿片类药物的母血浓度分布 妊娠期间和产后期间的肽。 以上 实验将能够评估可能的外围过程 参与妊娠引起的痛阈升高。 (6) 在 为了评估妊娠诱发的中枢机制的参与 镇痛母体脑脊液中 B-内啡肽的浓度分布， 将测定甲硫氨酸和亮氨酸脑啡肽以及总阿片类药物 妊娠期间。 (7) 上述操纵的影响和 模拟妊娠对母体血液和脑脊液中阿片类药物水平的影响 肽也将被研究。

The overall objective of the proposed research is to determine the peripheral and central processes that are responsible for the elevation in the threshold for responsiveness to aversive stimuli that is observed during gestation in rats. One approach that will be utilized will be to determine the effects of mimicking and manipulating specific aspects of the pregnant condition on the pain threshold in rats. A second approach will be to determine the materal blood and cerebrospinal fluid (CSF) concentration profile of opioid peptides during gestation. The specific objectives are to (1) Determine the role, if any, of estrogen, progesterone and prolactin, alone and in combination, on the pain threshold of nonpregnant ovariectomized rats. (2) Determine whether an elevation in pain threshold is observed during pseudopregnancy, with and without deciduoma. (3) Determine if an increase in intrauterine pressure and distention is sufficient to produce an increase in pain threshold in pseudopregnant rats and ovariectomized rats subjected to hormone treatment. (4) Determine whether the presence of a developing fetus(es) is essential for the elevation in maternal pain threshold. (5) Determine the maternal blood concentration profile of B-endorphin and total opioid peptides during gestation and the post partem period. The above experiments will enable the evaluation of peripheral processes that might be involved in the pregnancy-induced elevation in pain threshold. (6) In order to assess the involvement of central mechanisms in pregnancy-induced analgesia the maternal CSF concentration profile of B-endorphin, methionine- and leucine-enkephalin and total opioids will be determined during gestation. (7) The effects of the above manipulations and simulations of pregnancy on maternal blood and CSF levels of opioid peptides will also be investigated.